+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The lysosomal proton pump is electrogenic

, : The lysosomal proton pump is electrogenic. Journal of Biological Chemistry 258(17): 10403-10410

Lysosomes were purified approximately 40-fold from rat kidney cortex by differential and Percoll density gradient centrifugation. In a sucrose medium, the lysosomes quenched the fluorescence of the potential sensitive dye diS-C3-(5) (3,3'-dipropylthiocarbo-cyanine iodide) in a time-dependent manner, indicating that the dye accumulates within the lysosomal interior. After treatment of the lysosomes with valinomycin, the dye fluorescence displayed a logarithmic dependence upon the external K+ concentration; thus, the fluorescence signal provides a semiquantitative measure of the lysosomal membrane potential (delta psi). In the absence of valinomycin, lysosomal quenching of diS-C3-(5) fluorescence was partially reversed by agents which collapse the lysosomal pH gradient (ammonium sulfate, chloroquine, and K nigericin), suggesting that the proton gradient across the lysosomal membrane contributes to delta psi. A rapid increase in diS-C3-(5) fluorescence, indicative of an increase in delta psi, was observed upon the addition of Mg-ATP to the lysosomes. The ATP-dependent fluorescence change was inhibited by protonophores, K valinomycin, permeable anions, and N-ethylmaleimide, but was unaffected by ammonium sulfate, K nigericin, or sodium vanadate. Oligomycin had no effect at concentrations below 2 micrograms/ml; at higher concentrations, oligomycin partially inhibited the fluorescence response to Mg-ATP, but it also inhibited the fluorescence response to K valinomycin, suggesting that it had modified the permeability of the lysosomal membrane. Dicylohexylcarbodiimide behaved similarly to oligomycin. Mg-ATP also altered the lysosomal distribution of 86Rb+ (in the presence of valinomycin) and S[14C]CN-, consistent with an increase in the potential of the lysosomal interior of 40-50 mV. The results demonstrate that the lysosomal proton pump is electrogenic.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006712621

PMID: 6224789

DOI: 10.1055/s-2007-1018715

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Nie ZhenBang, 1999: Consideration of the adjustment and perfection of the structure of agricultural production in China. The recent and current changes in China, their effects on agricultural production and problems in the agricultural production structure are considered. Ways of improving the production structure are discussed including the continued introduction o...

Cragg B.A.; Fry J.C.; Thurley S.S.; Bacchus Z., 1981: The aquatic vegetation of llanbwchllyn reservoir powys wales uk. The study complements previous work on the aquatic vegetation [mostly macrophytes] of Llanbwchllyn and on attempts to assess the impact of the raising of the water level in 1975. The surveys reveal considerable changes in the distribution of aquat...

Vogel, K. P.; Masters, R. A.; Callahan, P. J.; Grams, K., 1998: A rotary seed processor for removing pubescence from seed of prairie grasses. A rotary seed processor was developed that efficiently processes small breeder or experimental lots of seed that can then be easily sown with small plot cone planters or conventional planters. The processor consists of a metal cylinder that is lin...

Mees, G. F., 1970: Notes on some birds from the Island of Formosa (Taiwan). Zool. Meded., Leiden, 44: 285-304

Sarasu, C.; Sivagamasundari, S., 1982: Hardening of the egg-shell of a pill-millipede Arthrosphaera lutescens (Diplopoda: Myriapoda). Indian Zoologist, 61-2: 73-75

Mugnier, Jean-Louis; Mascle, Georges; Faucher, Thierry, 1993: Structure of the Siwaliks of western Nepal; an intracontinental accretionary prism. International Geology Review 35(1): 1-16

Olin J.W.; Cressman M.D.; Young J.R.; Hoogwerf B.; Weinstein C.E., 1988: Lipoprotein abnormalities in patients with lower extremity arteriosclerosis obliterans. Arteriosclerosis 8(5): 581A

Carlson, Adriane M.; Gorchov, David L., 2002: Herbicide effects on Alliaria petiolata and subsequently on the plant community in Hueston Woods, OH. Ecological Society of America Annual Meeting Abstracts 87: 329

Ginns J., 1986: Oedocephalum cristallinum. Fungi Canadenses (304)

Chumakov K.M., 1980: A highly efficient method of cell infection with encephalomyocarditis virus rna. A method for infecting Krebs-II [mouse lymphoma] cells with encephalomyocarditis virus RNA based on treatment of the cells with DEAE-dextran at high concentration is elaborated. The specific infectivity of virion RNA was 3 .times. 108 plaque-formi...