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The macrophage membrane potential test an electro physiological modification of the field caspary test in melanoma patients


, : The macrophage membrane potential test an electro physiological modification of the field caspary test in melanoma patients. Acta Biologica et Medica Germanica 34(11-12): 1911-1914

In modification of the Field and Caspary cytopherometric test, the influence of the cell-free supernatant (lymphokine) from the lymphocyte antigenic reaction on the transmembrane potential of macrophages obtained from guinea pigs (peritoneal exudate cells [PEC]) was studied. Macrophage membrane potential (MMP) was determined by the glass microelectrode technique. In agreement with histological and clinical findings in malignant melanoma and malignant Dubreuilh's disease, a depolarization of the macrophage membrane of up to 40% was observed. A depolarization up to 60% was obtained in patients having undergone BCG vaccination, if the antigen used was PPD [purified protein derivative]. In control persons and in non-malignant pigmentary tumors (such as foreign body granuloma) no variation of MMP was found. Cell mediated immunity is probably closely correlated with membrane permeabilities, ion gradients and cell metabolism.

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Other references

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Bahna, S. L., 1994: Blind food challenge testing with wide-open eyes. A review. Because food allergy treatment is basically avoidance of the offending food(s), identification of the latter becomes the cornerstone for management. At present, the elimination-challenge test is the most definitive procedure for verifica...

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Ranau, Reiner; Oehlenschlaeger, Joerg; Steinhart, Hans, 2001: Aluminium content in edible parts of seafood. Samples of the edible parts of different fish species and of crustacean and molluscan shellfish were collected in the North Sea, the Barents Sea, the Baltic Sea, the Northeast Atlantic, and in Greenland waters. Most of the aluminium concentrations...

Edwards, Robin .; Broad, Gavin ., 2006: Provisional atlas of the aculeate Hymenoptera of Britain and Ireland. Part 6. Unknown

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Gutu M., 1971: Details of the rostrum in palaemon adspersus crustacea decapoda of the black sea considered as a taxonomic criterion. Travaux du Museum d'Histoire Naturelle "Grigore Antipa" 11: 83-90

Newbery, D M. .; Prins, HH T. .; Brown, N. D. ., 1998: Dynamics of tropical communities: the 37th symposium of the British Ecological Society, Cambridge University, 1996. The 22 papers in this book were presented at a symposium held at Cambridge University on 1-3 April 1996, which considered the problems confronting advancement of the understanding of the dynamics of tropical communities when there is stochasticity...