+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The magnesium dependent atpase from the erythrocyte plasma membrane of the flounder platichthys flesus general properties and some observations on the steady state kinetics

, : The magnesium dependent atpase from the erythrocyte plasma membrane of the flounder platichthys flesus general properties and some observations on the steady state kinetics. Comparative Biochemistry & Physiology B 75(1): 153-162

The plasma membrane of the flounder erythrocyte contains a Mg2+-dependent ATPase which is insensitive to ouabain Mg2+ is part of the substrate, Mg-ATP, and Mg2+ also functions as a nonessential activator. Ca2+, Mn2+ and Co2+ can replace Mg2+ as an activator of ATP hydrolysis. Cu2+ and Zn2+ abolish the Mg-dependent activity. Ca2+ and Mg2+ activate the same enzyme and Mg-ATP and Ca-ATP are mutually competitive. The hydrolysis of ATP obeys Michaelis-Menten kinetics whether or not the Mg2+-ATPase is fully activated by Mg2+. The Km values for Mg-ATP were 0.13 and 0.43 mM respectively. Free ATP acts as a competitive inhibitor towards Mg-ATP and the dissociation constant for the enzyme-ATP complex was .apprx. 0.55 mM. The Mg2+-ATPase has a low specificity and reacts with GTP, ITP, UTP and CTP. The enzyme has a broad pH optimum ranging from 6.5-7.2 and an energy of activation of 13.5 kcal/mol between C. The effect of some activators and inhibitors of membrane-bound ATPases are reported.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006712842

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Momii, K.; Otsuki, K.; Yano, T., 1995: Water requirements of drip-irrigated cabbage at the coastal arid lands in the California peninsula, Mexico. The irrigation water requirements of cabbage for maximum yield with minimum water use were investigated based on meteorological conditions and soil moisture dynamics at coastal areas in California peninsula, Mexico. Six rates of water application,...

Ayyangar, G. S., 1933: Differentiation of hairs on the seed coat of cotton. The presence of primordial lint cells and their stages of development, observed in microtome sections of Cambodia cotton ovules up to 7 days old, is adduced as evidence that the sprouting of hairs continues even after flowering. Changes in the nat...

Schoeller Henri, 1950: Les variations de la composition chimique de leau dans les nappes souterraines. Discusses variations in the chemical composition of ground waters within a single aquifer, or within different water-bearing formations of similar age and petrographic character in a single region, based on analyses of waters from Quaternary aquif...

Wolfe M.L.; Allen D.L., 1973: Continued studies of the status socialization and relationships of isle royale wolves 1967 to 1970. Journal of Mammalogy 54(3): 611-633

Jiang, Hongliang; Timmermann, Barbara, N.; Gang, David, R., 2007: Characterization and identification of diarylheptanoids in ginger (Zingiber officinale Rosc.) using high-performance liquid chromatography/electrospray ionization mass spectrometry. In our continuing investigation of diarylheptanoids in Zingiberaceae plants using liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS/MS), 26 diarylheptanoids were identified from fresh ginger rhizome. Of the 26 compounds,...

Zhou, Long Wu; Zhang, Sui Po; Connell, Thomas A.; Weiss, Benjamin, 1993: Cholinergic lesions of mouse striatum induced by AF64A alter D-2 dopaminergic behavior and reduce D-2 dopamine receptors and D-2 dopamine receptor mRNA. To determine whether dopamine receptors are expressed in acetylcholine-containing neurons intrinsic to the striatum, and to study further the interactions between the dopaminergic and cholinergic systems, the irreversibly acting cholinergic neurot...

Crawford, Ma; Hassam, Ag; Willis, Al; Stone, Jf, 1980: The key role of rate limitations in the elongation and desaturation of fatty acids for structural lipds and prostaglandins. Annales de la nutrition et de l'alimentation = Annals of nutrition and food4(2): 187

Cohen D.J., 1981: Sensory receptor sensitivity in autistic children response times to proximal and distal stimulation. On the basis of behavioral observation, it has been postulated that autistic children prefer proximal (touch, taste) to distal (sight, hearing) sensory modalities. To assess this systematically, autistic children's simple response times were...

Oldham David W., 1997: Exploration for shallow compaction-induced gas accumulations in sandstones of the Fort Union Formation, Powder River basin, Wyoming. The Mountain Geologist 34(1): 25-36

Green, W. W.; Stevens, W. R.; Gauch, M. B., 1971: Use of body measurements to predict the weights of wholesale cuts of beef carcasses : wholesale arm chuck of 1000 pound steers. 185 measurements were taken on 100 beef-type steers weighing from 1000 to 1050 Ib. Individual measurements and combinations thereof were used to obtain the best variables to predict the weight of the wholesale arm chuck cuts of the measured animal...