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The magnesium dependent atpase of bovine cardiac myosin and its subfragment 1


, : The magnesium dependent atpase of bovine cardiac myosin and its subfragment 1. Biochemical Journal 159(2): 301-316

The kinetics of the Mg2+-dependent ATPase activity of bovine cardiac myosin and its papain subfragment-1 were studied by using steady-state and pre-steady-state techniques, and results were compared with published values for the corresponding processes in the ATPase mechanism of rabbit skeletal-muscle myosin subfragment-1. The catalytic-center activity for cardiac subfragment-1 was 0.019 s-1, which was < 1/3 of that determined for the rabbit protein. The ATP-induced isomerization process, measured from enhancement of protein fluorescence on substrate binding, was similarly decreased in rate, as was the isomerization process associated with ADP release. However, the equilibrium constant for ATP cleavage, measured by quenched-flow by using [.gamma.-32P]ATP, showed little difference in the 2 species. Other experiments were carried out to investigate the rate of association of actin with subfragment-1 by light-scattering changes and also the rate of dissociation of the complex by ATP. The dissociation rate increased with increasing substrate concentration, to a maximum at high ATP concentrations, with a rate constant of about 2000 s-1. Isomerization processes which may involve conformational changes probably have substantially lower rate constants for the cardiac proteins, whereas equilibrium constants for substrate binding and cleavage are not significantly different. These differences may be related to the functional properties of these myosins in their different muscle types. Kinetic heterogeneity was detected in both steady-state and transient processes, and this is discussed in relation to the apparent chemical homogeneity of cardiac myosin.

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Other references

Stanton Hicks M., 1977: Intercostal block and ventilatory function following upper abdominal surgery. Upsala Journal Of Medical Sciences Supplement: 42

BOSHES, L.D., 1961: The challenge of parkinsonism. Illinois Medical Journal 120: 336-337

Penny, D. H., 1973: The Ciawi Training Center. A case study in the teaching of social science research methods. The Ciawi Training Centre, Indonesia, plays an important part in furthering the work of the agro-economic survey of Indonesia. Its main value is to illuminate the nature of the 'research process'. It also shows that the best and quickest...

Takeuchi, A.; Okano, T.; Sayamoto, M.; Sawamura, S.; Kobayashi, T.; Motosugi, M.; Yamakawa, T., 1986: Tissue distribution of 7-dehydrocholesterol, vitamin D3 and 25-hydroxyvitamin D3 in several species of fishes. A high-performance liquid chromatographic (HPLC) method for simultaneous determination of 7-dehydrocholesterol (7-DHC), vitamin D3 and 25-hydroxyvitamin D3 (25-OH-D3) in tissues of fishes was established, and using this method the tissue distribut...

Ellenbogen K.A., 1984: Comparison of cryoablation of the atrioventricular node vs catheter ablation of the his bundle. American Heart Association Monograph: Ii-412

Gundorova, R.A.; Ziangirova, G.G.; Chudnaia, N.I., 1970: Use of low temperatures to remove tumors of the eye. Oftalmologicheskii Zhurnal 25(1): 49-52

Belyakov L. N., 1975: Foraminifera and Carboniferous paleogeography in the extreme northeast European part of the USSR. Voporsy Mikropaleontologii 18): 51-55

Morimoto, Y., 2016: Continuous interscalene brachial plexus block. Acta Anaesthesiologica Taiwanica 54(2): 75-75

Elliott, S.A.; Van Auken, O.W., 1997: Competition between an annual Compositae and a perennial grass. Bulletin of the Ecological Society of America 78(4 SUPPL ): 83

Schimmer, R.C.; Brulhart, K.B.; Duff, C.; Glinz, W., 1998: Arthoscopic partial meniscectomy: A 12-year follow-up and two-step evaluation of the long-term course. The long-term results after arthroscopic partial meniscectomy of 119 patients with a mean follow-up of 12 years are presented in this study. The same series of patients had an earlier follow-up 4 years postoperatively. Thus, an evaluation of the a...