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The mechanism of dextransucrase action. Activation of dextransucrase from Streptococcus mutans OMZ 176 by dextran and modified dextran and the nonexistence of the primar requirement for the synthesis of dextran



The mechanism of dextransucrase action. Activation of dextransucrase from Streptococcus mutans OMZ 176 by dextran and modified dextran and the nonexistence of the primar requirement for the synthesis of dextran



Archives of Biochemistry and Biophysics 183(2): 726-731



S. mutans OMZ 176 was grown in a sucrose-free medium containing fructose as a carbohydrate source. Dextransucrase was precipitated from the culture supernatant with 40% saturated ammonium sulfate. The activity of dextransucrase apparently was stimulated by exogenous dextran. Maximum activity was reached when concentration of exogenous dextran was 2 mg/ml. Dextrans modified at nonreducing ends by reaction with tripsyl chloride and/or by hydrolysis with an exodextranase activated dextransucrase 4-6 times that of a control. Exodextranase-modified dextrans had nonreducing chains that were very short in comparison with unmodified dextran, and the tripsyl-modified dextrans had chains that were blocked at nonreducing ends with a triisopropylbenzenesulfonyl group on the C6 hydroxyl group. Because nonreducing ends of modified dextrans were not available for reaction, activation of dextransucrase by these modified dextrans could not be due to primer reactions with nonreducing ends. Activation of dextransucrase, thus, must be by an alternate mechanism. Two alternative mechanisms discussed were an allosteric effect and nucleophilic displacement reactions by the added dextran. Addition of increasing amounts of dextran apparently shifted the synthesis from an insoluble dextran to a soluble dextran.

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Accession: 006715420

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PMID: 921284


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