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The metabolically active pool of uracil in euglena gracilis principle of measurement and its role in light induced rna synthesis


Biochemie und Physiologie der Pflanzen (BPP) 178(6-7): 529-545
The metabolically active pool of uracil in euglena gracilis principle of measurement and its role in light induced rna synthesis
In E. gracilis uracil was compartmentalized in a metabolically active pool operating in the anabolic reactions to feed RNA synthesis and the uracil degradation and in a storage pool. Using [5-3H]-uracil incorporation into acid-insoluble material or 14CO2 release from [2-14C]-uracil, distinct lag times in the labeling of both products were determined by 6 different types of tracer kinetics as a measure of the metabolic pool size. This size was 11 pmoles/107 cells in heterotrophically grown cells. The metabolically active pool of uracil which increased by preloading with uracil in a time-dependent reversible and saturable process seems to be of functional significance during high rate of RNA synthesis. Its size decreased to almost zero during light-induced RNA synthesis, was growth-phase dependent in different cell types and apoplastic mutants and was undetectable in autotrophically grown cells.


Accession: 006717235



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