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The metabolically active pool of uracil in euglena gracilis principle of measurement and its role in light induced rna synthesis



The metabolically active pool of uracil in euglena gracilis principle of measurement and its role in light induced rna synthesis



Biochemie und Physiologie der Pflanzen (BPP) 178(6-7): 529-545



In E. gracilis uracil was compartmentalized in a metabolically active pool operating in the anabolic reactions to feed RNA synthesis and the uracil degradation and in a storage pool. Using [5-3H]-uracil incorporation into acid-insoluble material or 14CO2 release from [2-14C]-uracil, distinct lag times in the labeling of both products were determined by 6 different types of tracer kinetics as a measure of the metabolic pool size. This size was 11 pmoles/107 cells in heterotrophically grown cells. The metabolically active pool of uracil which increased by preloading with uracil in a time-dependent reversible and saturable process seems to be of functional significance during high rate of RNA synthesis. Its size decreased to almost zero during light-induced RNA synthesis, was growth-phase dependent in different cell types and apoplastic mutants and was undetectable in autotrophically grown cells.

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