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The metabolism of 3 tritium labeled carbon 14 labeled oleanolic acid 3 o monoglucoside in isolated cells from calendula officinalis leaves



The metabolism of 3 tritium labeled carbon 14 labeled oleanolic acid 3 o monoglucoside in isolated cells from calendula officinalis leaves



Phytochemistry 24(8): 1713-1716



The radioactive precursor, [3-3H]oleanolic acid-3-O-mono-[14C]glucoside was administered to isolated cells obtained from the leaves of C. officinalis. The radioactivity of the precursor was incorporated into fractions containing free oleanolic acid, individual glucosides, glucuronide F and other glucuronides. The ratio of 3H:14C radioactivity in these fractions indicated that glucosides were formed in a process involving direct glycosylation of the precursor, whereas the glucuronides were formed from oleanolic acid released by hydrolysis of the precursor. Dynamics curves showed that glucoside II formed by direct glycosylation of the precursor was intensively transformed to other derivatives.

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Accession: 006717340

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