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The mitochondrial and cytosolic forms of avian phosphoenol pyruvate carboxy kinase gtp ec 4.1.1.32 are encoded by different messenger rna species



The mitochondrial and cytosolic forms of avian phosphoenol pyruvate carboxy kinase gtp ec 4.1.1.32 are encoded by different messenger rna species



Journal of Biological Chemistry 257(22): 13787-13794



Previous work indicated that in the chicken, hepatic phosphoenolpyruvate carboxykinase is in the mitochondria, whereas kidney contains both a mitochondrial and cytosolic form of the enzyme. Here, the 2 forms of phosphoenolpyruvate carboxykinase were purified and shown to be distinct proteins which differ in size, charge and immunochemical properties. In a [rabbit reticulocyte] cell-free protein synthesis system, the cytosolic isozyme was encoded only by kidney mRNA and its translation form had similar properties to that of the mature protein. The mitochondrial enzyme was encoded by liver and kidney mRNA and synthesized as a protein .apprx. 2000 daltons larger than the mature form. The putative precursor of the mitochondrial phosphoenolpyruvate carboxykinase was processed to a mature size by isolated, respiring mitochondria. The mRNA species for the cytosolic and mitochondrial forms are separable and are .apprx. 3 and 4 kilobases, respectively. The 2 forms of phosphoenolpyruvate carboxykinase of the chicken are apparently encoded by distinct mRNA species.

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