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The mitochondrial and cytosolic forms of avian phosphoenol pyruvate carboxy kinase gtp ec 4.1.1.32 are encoded by different messenger rna species


, : The mitochondrial and cytosolic forms of avian phosphoenol pyruvate carboxy kinase gtp ec 4.1.1.32 are encoded by different messenger rna species. Journal of Biological Chemistry 257(22): 13787-13794

Previous work indicated that in the chicken, hepatic phosphoenolpyruvate carboxykinase is in the mitochondria, whereas kidney contains both a mitochondrial and cytosolic form of the enzyme. Here, the 2 forms of phosphoenolpyruvate carboxykinase were purified and shown to be distinct proteins which differ in size, charge and immunochemical properties. In a [rabbit reticulocyte] cell-free protein synthesis system, the cytosolic isozyme was encoded only by kidney mRNA and its translation form had similar properties to that of the mature protein. The mitochondrial enzyme was encoded by liver and kidney mRNA and synthesized as a protein .apprx. 2000 daltons larger than the mature form. The putative precursor of the mitochondrial phosphoenolpyruvate carboxykinase was processed to a mature size by isolated, respiring mitochondria. The mRNA species for the cytosolic and mitochondrial forms are separable and are .apprx. 3 and 4 kilobases, respectively. The 2 forms of phosphoenolpyruvate carboxykinase of the chicken are apparently encoded by distinct mRNA species.

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Related references

Hod Y.; Yoo Warren H.; Morris S.; Hanson R.W., 1983: Separate genes code for the cytosolic and mitochondrial forms of phosphoenol pyruvate carboxy kinase. Federation Proceedings 42(7): ABSTRACT 14

Chuang D.T., 1978: Structural comparison of the mitochondrial and cytosolic forms of phosphoenol pyruvate carboxy kinase from monkey liver. Federation Proceedings 37(6): 1798

Cooper, T.G.; Tchen, T.T.; Wood, H.G.; Benedict, C.R., 1968: The carboxylation of phosphoenol pyruvate and pyruvate i the active species of carbon di oxide utilized by enz phosphoenol pyruvate carboxy kinase enz carboxy trans phosphorylase and enz pyruvate carboxylase rhodospirillum rubrum propionibacterium shermanii. Journal of Biological Chemistry 243(14): 3857-3863

Ballard, F.J.; Hanson, R.W., 1969: Purification of phosphoenol pyruvate carboxy kinase from the cytosol fraction of rat liver and the immunochemical demonstration of differences between this enzyme and the mitochondrial phosphoenol pyruvate carboxy kinase. Journal of Biological Chemistry 244(20): 5625-5630

Colin W.W.; Schofield P.J.; Johnstone I.L., 1968: Carbon di oxide fixation in haemonchus contortus larvae enz phosphoenol pyruvate carboxy kinase enz malic enz phosphoenol pyruvate carboxylase enz phosphoenol pyruvate carboxy trans phosphorylase. Comparative Biochemistry & Physiology 26(2): 537-544

Hod Y.; Utter M.F., 1981: Messenger rna from the chicken codes for 2 forms of phosphoenol pyruvate carboxy kinase. Federation Proceedings 40(6): 1574

Mencher, D.; Cohen, H.; Benvenisty, N.; Meyuhas, O.; Reshef, L., 1984: Primary activation of cytosolic phosphoenol pyruvate carboxy kinase ec 4.1.1.32 gene in fetal rat liver and the biogenesis of its messenger rna. The primary appearance of phosphoenolpyruvate (P-pyruvate) carboxykinase RNA transcripts in fetal liver was induced by a number of different stimuli. This may occur as rapidly as an hour after injection in utero of N6,O2'-dibutyryl-cAMP (Bt2c...

Watford M., 1981: The separate roles of cytosolic and mitochondrial phosphoenol pyruvate carboxy kinase enzymes in gluconeogenesis. Federation Proceedings 40(6): 1623

Goto, Y.; Ohki, Y.; Shimizu, J.; Shukuya, R., 1981: Cytosolic and mitochondrial phosphoenol pyruvate carboxy kinase ec 4.1.1.32 of the bull frog rana catesbeiana liver. The mitochondrial and cytosolic phosphoenolpyruvate carboxykinase (GTP: oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32) occurring in the bullfrog (R. catesbeiana) liver were studied. The enzymes in the 2 intracellular compartments...

Ogata K.; Watford M.; Brady L.J.; Hanson R.W., 1982: Mitochondrial phosphoenol pyruvate carboxy kinase gtp and the regulation on gluconeogenesis and ketogenesis in avian liver. Chicken liver synthesizes glucose from lactate and dihydroxyacetone at high rates but pyruvate, glycerol, alanine and other amino acids are poor glucose precursors. Despite its limited conversion to glucose, 1 mM pyruvate completely suppressed ket...