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The mitochondrial atp of acanthamoeba castellanii partial characterization and changes in activity during exponential growth


, : The mitochondrial atp of acanthamoeba castellanii partial characterization and changes in activity during exponential growth. Comparative Biochemistry & Physiology B 71(3): 495-500

The mitochondrial ATPase of A. castellanii is Mg2+-requiring (optimum cation: ATP ratio of 1.5) and has 2 pH optima of activity (at pH 6.6 and 8.1). ATPase activity of submitochondrial particles is effectively inhibited by 12 different inhibitors of energy conservation suggesting similarities in inhibitor-binding sites to other previously characterized complexes. Gel filtration by passage through Sephadex G-50 increases ATPase activity of submitochondrial particles between 1.5-3.5 fold indicating the presence of a low MW inhibitor protein. After removal of the inhibitor protein, sensitivity to inhibitors of energy conservation decreases by between 1.5-14 fold. Crude F1-inhibitor preparations from A. castellanii, Schizosaccharomyces pombe, Tetranymena pyriformis and bovine heart also inhibit ATPase activity. Large variations in ATPase activity, F1-inhibitor protein activity and amounts of immunologically-determined ATPase protein were observed during exponential growth and the correlation between changes in these measurements is discussed. The results are also discussed highlighting the similarities between the mitochondrial ATPase of A. castellanii and other mitochondrial ATPases.

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Filgueiras, E. P.; Hossain, S. M.; Bertechini, A. G.; Oliveira, A. I. G. de, 1997: Bioavailability of copper sulfate and copper oxide for piglets from 10 to 25 kg. The effects of supplementation of the basal diet (consisting of maize and soyabean meal with 18% crude protein, 3400 kcal DE/kg, 0.7% Ca, 0.6% total P and 9.6 ppm of copper) with 0, 100, 200 or 400 ppm of copper from copper sulfate (25.4%), copper...

Tsuchimochi H.; Kuro O.M.; Yoshida K.; Kawana M.; Kimata S I.; Yazaki Y., 1986: Expression of myosin isozymes during the developmental stage and their redistribution induced by pressure overload. Cardiac muscles contain at least two isozymes.sbd.referred to as .alpha.(HC.alpha.) and .beta.(HC.beta.).sbd.of the myosin heavy chain. The proportional ratio of these isozymes varies depending upon the developmental stage and the physiological an...

Dolan W.D.; Gifford R.W.Jr; Smith R.J.; Beljan J.R.; Cooper T.; Friedlander I.R.; Kastan M.B.; Moxley J.H.IIi; Schmidt R.T.F., 1982: Continuous ambulatory peritoneal dialysis. Patients (11) were treated with continuous ambulatory peritoneal dialysis (CAPD) for a cumulative treatment duration of 72 mo. The patients had higher Hb and hematocrit levels and were generally in better clinical condition than most patients trea...

Fisher, R.; Waller, E. K.; Grossi, G.; Thompson, D.; Tizard, R.; Schleuning, W. D., 1985: Isolation and characterization of the human tissue type plasminogen activator structural gene including its 5' flanking region. mRNA specific for tissue type plasminogen activator (t-PA) is induced in HeLa cells by the tumor promoter phorbol myristate acetate (Waller, E. K., and Schleuning, W. D. (1985) J. Biol. Chem. 260, 6354-6360). To study the underlying mechanism, a c...

Drigalenko, E., 1999: Matrix representation of the Haseman-Elston method. The Haseman-Elston method is a means of detecting linkage between loci on the basis of the probability of the members of a sib pair having 0, 1 or 2 alleles that are identical by descent at a marker and a trait locus. In the present work these pro...

Augustyniak, Patrick, N.; Kourrich, Said; Rezazadeh, Shohreh, M.; Stewart, Jane; Arvanitogiannis, Andreas, 2006: Differential behavioral and neurochemical effects of cocaine after early exposure to methylphenidate in an animal model of attention deficit hyperactivity disorder. We used a putative animal model of attention deficit hyperactivity disorder (ADHD), the SHR rat, to examine the effects of repeated exposure to methylphenidate (MPH; Ritalin (R)) during the pubertal period on cocaine-induced conditioned place pref...

Mussgay, M.; Dietzschold, B.; Frenzel, B.; Kaaden, O. R.; Straub, O. C.; Weiland, F., 1977: The bovine leukosis virus. Medical Microbiology and Immunology 164(1/3): 131-138

Timme, Sl; Bowers, Fd, 1988: Pleurochaete squarrosa (Brid.) Lindb. new to Mississippi, and extensions of its range in Alabama. Bryologist er 91(4): 368-369

Candela, Monica, G.; Barbera, Gonzalo, G.; Sallent, Angel; Leon, Luis, 2008: Microbiological survey for selected bacterial pathogens in European storm petrel Hydrobates pelagicus, Linnaeus 1758 from Grosa Island Murcia, Southeastern Spain. The current work shows the first step in the knowledge on the health status of European storm petrel (Hydrobates pelagicus) colony inhabiting Grosa Island (Murcia, SE Spain). We performed a screening about the bacterial pathogens carried by them...

Moore, K., 1983: Grass dwarfing genes as a source of reduced height and increased tillering in wheat (Triticum aestivum L.). Proceedings of the sixth International Wheat Genetics Symposium edited by Sadao Sakamoto: 646