EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The mitochondrial voltage dependent channel voltage dependent anion selective channel is modified asymmetrically by succinic anhydride


, : The mitochondrial voltage dependent channel voltage dependent anion selective channel is modified asymmetrically by succinic anhydride. Journal of Membrane Biology 83(1-2): 87-94

Succinic anhydride was previously shown to react with the outer mitochondrial membrane channel-forming protein, VDAC [voltage dependent anion-selective channel], resulting in the loss of its voltage dependence. The anhydride was presently added to VDAC held in a particular conformational state by means of an applied electric field. VDAC was inserted into the membranes from the cis side and the anhydride was added either to the cis or trans side. Channels modified in the open state behaved similarly whether anhydride was added to the cis or trans side. Modifications of VDAC in either of the 2 closed states did not. Modifications resulting in the loss of voltage-dependence occurred primarily when anhydride was added to the negative side of the membrane irrespective of which closed state the VDAC was in indicating that the accessibility of the gating charges alternated between the cis and trans sides as the channel's conformation was changed from one closed state to the other. Despite the pronounced asymmetry, in general the resulting channels behaved in the same way in response to either positive or negative fields. A model consistent with the results is presented which proposes that the same gating charges are responsible for channel closure at both positive and negative fields.


Accession: 006719119

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Doring C.M.C., 1985: Voltage dependence and ion selectivity of the mitochondrial channel voltage dependent anion selective channel are modified by succinic anhydride. The mitochondrial protein VDAC [voltage dependent anion-selective channel] forms voltage-dependent anion-selective channels in planar phospholipid membranes. When succinic anhydride was added to these membranes, it virtually eliminated VDAC's...

Doring, C.; Colombini, M., 1985: The mitochondrial voltage-dependent channel, VDAC, is modified asymmetrically by succinic anhydride. In the accompanying paper, succinic anhydride was shown to react with the outer mitochondrial membrane channel-forming protein, VDAC, resulting in the loss of its voltage dependence. In this paper, the anhydride was added to VDAC held in a particu...

Colombini M., 1983: Simultaneous alteration of ion selectivity and voltage dependence by reacting the channel former voltage dependent anion selective channel with succinic anhydride. Biophysical Journalpart 2: 48a

Bowen K.A.; Tam K.; Colombini M., 1985: Evidence for titratable gating charges controlling the voltage dependence of the outer mitochondrial membrane channel voltage dependent anion selective channel. A voltage-dependent anoin-selective channel, VDAC [rat], is found in outer mitochondrial membranes. VDAC's conductance is known to decrease as the transmembrane voltage is increased in either the positive or negative direction. Charged groups...

Doring C.; Colombini M., 1984: On the nature of the molecular mechanism underlying the voltage dependence of the channel forming protein voltage dependent anion selective channel. Biophysical Journal 45(1): 44-46

Doring, C.; Colombini, M., 1984: On the Nature of the Molecular Mechanism Underlying the Voltage Dependence of the Channel-Forming Protein, Voltage-Dependent Anion-Selective Channel (VDAC). Biophysical Journal 45(1): 44-46

Báthori, Görgy.; Csordás, Görgy.; Garcia-Perez, C.; Davies, E.; Hajnóczky, Görgy., 2006: Ca2+-dependent control of the permeability properties of the mitochondrial outer membrane and voltage-dependent anion-selective channel (VDAC). Cell function depends on the distribution of cytosolic and mitochondrial factors across the outer mitochondrial membrane (OMM). Passage of metabolites through the OMM has been attributed to the voltage-dependent anion-selective channel (VDAC), whi...

Baathori, G.; Csordaas, G.; Garcia-Perez, C.; Davies, E.; Hajnaoczky, G., 2006: Ca-dependent Control of the Permeability Properties of the Mitochondrial Outer Membrane and Voltage-dependent Anion-selective Channel (VDAC). Cell function depends on the distribution of cytosolic and mitochondrial factors across the outer mitochondrial membrane (OMM). Passage of metabolites through the OMM has been attributed to the voltage-dependent anion-selective channel (VDAC), whi...

Doring, C.; Colombini, M., 1985: Voltage dependence and ion selectivity of the mitochondrial channel, VDAC, are modified by succinic anhydride. The mitochondrial protein VDAC forms voltage-dependent anion-selective channels in planar phospholipid membranes. When succinic anhydride was added to these membranes, it virtually eliminated VDAC's voltage-dependence and changed its selectiv...

Colombini M., 1979: Properties of a voltage dependent anion selective channel in the outer mitochondrial membrane. Biophysical Journalpart 2: 46