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The mitogenic principle of escherichia coli lipo protein bone marrow derived lymphocyte mitogenicity n palmitoyl cysteine and n palmitoyl glutamic acid alpha methyl esters


, : The mitogenic principle of escherichia coli lipo protein bone marrow derived lymphocyte mitogenicity n palmitoyl cysteine and n palmitoyl glutamic acid alpha methyl esters. Biochemical & Biophysical Research Communications 92(4): 1389-1396

N-Palmitoyl-cysteine methyl ester and N-palmitoyl-glutamic acid .alpha.-methyl ester, which are analogs to the lipophilic N-terminal part of the lipoprotein from the outer membrane of E. coli, were synthesized and tested for biological activity in an in vitro lymphocyte culture system: in spleen cells from several inbred mouse strains the fatty acid derivatives exhibited mitogenic activity towards B [bone marrow derived] lymphocytes comparable to the effect of lipoprotein, as measured by 3H-thymidine incorporation and by hemolytic plaque assays. These results confirm former investigations, which showed that the mitogenic principle of the lipoprotein molecule resides in its N-terminal fatty acid-containing part. The proper dispersion of the water-insoluble substances was critical for their mitogenic activity. Optimal mitogenicity was obtained by sonicating the substances at concentrations of 0.1 mg/ml in culture medium.

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Related references

Cybulla, J.; Brückner, H.; Jung, G.; Wipperfürth, T.; Bessler, W.G., 1980: The mitogenic principle of Escherichia coli lipoprotein: B-lymphocyte mitogenicity of N-palmitoyl-cysteine and N-palmitoyl-glutamic acid alpha-methyl ester. Biochemical and Biophysical Research Communications 92(4): 1389-1396

Jung G.; Carrera C.; Brueckner H.; Bessler W.G., 1983: The mitogenic principle of escherichia coli lipo protein synthesis spectroscopic characterization and mitogenicity of n palmitoyl s 2 r s 2 3 dipalmitoyloxypropyl r cysteine methyl ester. The lipophilic N-terminal part of the lipoprotein from the outer membrane of E. coli, N-palmitoyl-S-[(2R,S)-2,3-dipalmitoyloxypropyl]-(R)-cysteine, was synthesized via diacylation of L-cystine dimethyl ester, reduction to N-palmitoyl-L-cysteine me...

Bessler W.G.; Cox M.; Wiesmueller K.H.; Jung G., 1984: The mitogenic principle of escherichia coli lipo protein b lymphocyte mitogenicity of the synthetic analog palmitoyl tetra peptide. N-Palmitoyl-(S)-seryl-(S)-seryl-(S)-asparaginyl-(S)-alanine (palmitoyl-tetrapeptide) is an analog of the N-terminal part of the lipoprotein from the outer membrane of E. coli. It was prepared by chemical synthesis and tested for biological activit...

Bessler, W.G.; Cox, M.; Wiesmüller, K.H.; Jung, G., 1984: The mitogenic principle of Escherichia coli lipoprotein: B-lymphocyte mitogenicity of the synthetic analogue palmitoyl-tetrapeptide (Pam-Ser-Ser-Asn-Ala). N-Palmitoyl-(S)-seryl-(S)-seryl-(S)-asparaginyl-(S)-alanine (Palmitoyl-tetrapeptide) is an analogue of the N-terminal part of the lipoprotein from the outer membrane of Escherichia coli. It was prepared by chemical synthesis and tested for biologi...

Wiesmueller K H.; Bessler W.; Jung G., 1983: Synthesis of mitogenic s 2 3 bis palmitoyloxypropyl n palmitoyl penta peptide from escherichia coli lipo protein. The N-terminal pentapeptide of the lipoprotein from the outer membrane of E. coli was obtained by coupling S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-N-palmitoyl-(R)-cysteine to O-tert-butylseryl-O-tert-butylseryl-asparaginyl-alanine tert-butyl ester,...

Melchers, F.; Braun, V.; Galanos, C., 1975: The lipo protein of the outer membrane of escherichia coli a bone marrow derived lymphocyte mitogen. Journal of Experimental Medicine 142(2): 473-482

Bessler W.G.; Von Tittle T., 1983: Synthetic analogs of bacterial lipo protein mitogenicity of tri palmitoyl penta peptide towards mouse lymphocytes and the bcl 1 lymphoid cell line. Immunobiology 165(3-4): 240

Bessler W.G.; Henning U., 1979: Protein i and protein ii from the outer membrane of escherichia coli are mouse bone marrow derived lymphocyte mitogens. Protein I from the outer E. coli membrane is a B [bone marrow-derived] lymphocyte mitogen in mice. Polyclonal mouse splenocytes activation was demonstrated by 3H-thymidine incorporation into DNA, 3H-uridine incorporation into RNA and by a hemolyti...

Gery I., 1975: Selective suppression of spleen bone marrow derived lymphocytes following treatment with escherichia coli lipo protein poly saccharide containing adjuvants. Israel Journal Of Medical Sciences: 1383-1384

Andersson, J.; Melchers, F.; Galanos, C.; Luederitz, O., 1973: The mitogenic effect of lipo poly saccharide on bone marrow derived mouse lymphocytes lipid a as the mitogenic part of the molecule. Journal of Experimental Medicine 137(4): 943-953