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The mobilities of granulocytes and the chemo tactic factor production by human mononuclear cells

, : The mobilities of granulocytes and the chemo tactic factor production by human mononuclear cells. Hokkaido Journal of Medical Science 56(2): 217-231

Various types of granulocyte mobilities and chemotactic factor production by human mononuclear cells were studied by the modified agarose plate methods. Three types of granulocyte mobilities, i.e., random mobility, chemotaxis and chemokinesis, are easily measured by the agarose plate method. Enhanced granulocyte random mobility by chemotactic factor was observed even when no gradient of chemotactic factor existed. This chemokinetic response decreased in cord blood leukocytes, which had a decreased chemotactic response compared to normal control adults. Not only chemotaxis but also chemokinesis may play an important role in inflammatory reactions in vivo. The degree of disturbance of chemotactic response of cord blood leukocytes which was observed in the agarose plate method was not so apparent as that which was reported by using the Boyden chamber method. The deformability of granulocytes probably contributed to the results of chemotaxis in the Boyden chamber method. When zymosan activated serum was used as the chemoattractant, the chemotactic response of cord blood leukocytes was more impaired than when Escherichia coli-derived factor was used. The same results were obtained when the chemotactic response of granulocytes from pediatric patients with malignancies were examined. Various chemotactic factors evidently should be used as the chemoattractant when the chemotaxis of granulocytes in any disease is examined. One of the chemotactic factors for granulocytes is cell-derived chemotactic factor for granulocytes. Production of chemotactic factor for granulocytes by human mononuclear cells was studied in the agarose plate method. It was designated as the chemotactic factor for granulocytes, CFG. CFG was produced by human mononuclear cells when they were stimulated with LPS lipopolysaccharide or anti-.beta.2-microglobulin. CFG producing cells were glass adherent and carbonyl Fe phagocytosing cells. CFG producing cells probably were monocytes. This CFG could not attract monocytes, although zymosan activated serum could attract both monocytes and granulocytes. Anti-human [complement] C5 serum could not abrogate the chemotactic activity of CFG. CFG evidently was different from C-derived chemotactic factor, C5a. Specific antiserum against human C3 or IgG could not abrogate the chemotactic activity of CFG.

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