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The mode of separation of chloroplast genomes in parasexual hybrid calli 2. heterogeneous distribution of 2 kinds of chloroplast genomes in hybrid callus


, : The mode of separation of chloroplast genomes in parasexual hybrid calli 2. heterogeneous distribution of 2 kinds of chloroplast genomes in hybrid callus. Plant Science Letters 32(1-2): 95-100

Parasexual hybrid callus (Nicotiana glauca + N. langsdorffii) was separated into 8 pieces by marking the relative position of each piece in an original callus, and Fraction I protein in each piece was analyzed by isoelectrofocusing. The ratios of 2 kinds of large subunits, which are coded in chloroplast DNA, are completely different in different positions in the callus. A hybrid callus is apparently a 3-dimensional mosaic of cells containing different ratios of 2 kinds of chloroplasts. The Fraction I protein of 5 sub-cloned calli grown from single cells isolated from the hybrid callus was analyzed. Two calli had only the N. langsdorffii type of large subunits and 1 callus had only the N. glauca type. Two calli contained both types of large subunits, showing that some cells in the original hybrid callus contained chloroplasts from both sources.

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Related references

Akada, S.; Hirai, A., 1983: Studies on the mode of separation of chloroplast genomes in parasexual hybrid calli. II. Heterogeneous distribution of two kinds of chloroplast genomes in hybrid callus. Parasexual hybrid callus (Nicotiana glauca + N. langsdorffii) was separated into eight pieces and the ribulose 1,5-diphosphate carboxylase in each piece was analysed by isoelectrofocusing. The results showed that the ratios of two kinds of large s...

Akada, S.; Hirai, A., 1986: Studies on the mode of separation of chloroplast genomes in parasexual hybrid calli iii. random separation of two types of chloroplast genomes in a hybrid callus. In the parasexual hybrid callus between Nicotiana glacua and N. langsdorffii, the two parental types of chloroplasts separate during callus development. The system to investigate the mode of separation involves the hormone independent growth of th...

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Hirai, A.A.ada, S.S.giura, S., 1988: Analysis of chloroplast genomes in parasexual hybrid calli. Applications of plant cell and tissue culture: 122

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