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The molecular basis for the reversed root effect in the blood of the marine prosobranch buccinum undatum


, : The molecular basis for the reversed root effect in the blood of the marine prosobranch buccinum undatum. Molecular Physiology 1(4): 209-212

The mechanism of the reversed Root effect is an allosteric ligand interaction between Cl-, CO2 and H+ on the O2-binding sites of the respiratory pigment. Approximately 70% of the O2-binding sites of B. undatum hemocyanin are blocked at high Cl- concentration (700 mM). Low Cl- concentration (300 mM) increases the number of binding sites involved in O2 binding by as much as 48%, when blood PCO2 is increased, and pH is decreased from .apprx. 8.0-7.4.

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