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The most probable number method for enumerating infective propagules of vesicular arbuscular mycorrhizal fungi in soil



The most probable number method for enumerating infective propagules of vesicular arbuscular mycorrhizal fungi in soil



Australian Journal of Soil Research 17(3): 515-519



Estimates of the number of infective propagules of vesicular-arbuscular (VA) endophytes in 2 soils were obtained using a most probable number (MPN) method. These estimates were compared with counts of the number of spores in the same soils obtained using a conventional wet sieving method. In 1 soil, there was good agreement between the number of coarse endophyte propagules, estimated by the MPN technique, and the number of germinable spores extracted using the wet sieving technique. A large population of fine endophyte propagules (more than 230/50 g soil) could only be enumerated using the MPN technique. In the 2nd soil, fewer coarse endophyte propagules were found using the wet sieving technique than when using the MPN technique. The MPN technique apparently gives a more realistic estimate of the number of infective propagules of VA endophytes in field soils than the conventional method.

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Accession: 006721222

Download citation: RISBibTeXText

DOI: 10.1071/sr9790515



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