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The multiple forms and kinetic properties of the n acetyl beta d hexosaminidases from colonic tumors and mucosa of rats treated with 1 2 di methyl hydrazine



The multiple forms and kinetic properties of the n acetyl beta d hexosaminidases from colonic tumors and mucosa of rats treated with 1 2 di methyl hydrazine



Biochemical Journal 177(1): 319-330



The separation and purification of the N-acetyl-.beta.-D-hexosaminidase activities from tumors induced by 1,2-dimethylhydrazine in the rat colon and from colonic mucosa of tumor-bearing animals are reported. Mucosa contained N-acetylhexosaminidases A and B, as well as a 3rd form whose properties with regard to electrophoretic mobility and thermostability lay between those of A and B. Tumors contained only N-acetylhexosaminidase A and B activities. Each form possessed both N-acetylglucosaminidase (EC 3.2.1.30) and N-acetlygalactosaminidase (EC 3.2.1.53) activities, which could not be separated by a variety of techniques. The alteration of the ratio of the 2 specific activities in each form during purification, together with differences in the kinetic inhibition constants and behavior during inactivation by various reagents or a temperature of 50.degree. C, supported the belief that each form contains the 2 enzyme activities, glucosaminidase and galactosaminidase, at separate active sites. This model is in contrast with that reported for these activities from a number of other sources. A variety of treatments reported to cause the conversion of form A into a form resembling B failed to produce such an effect on the rat colonic hexosaminidases.

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Accession: 006721656

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PMID: 34391


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