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The neuraminidase of human parainfluenza 1 virus ha 2 strain



The neuraminidase of human parainfluenza 1 virus ha 2 strain



Journal of General Virology 37(3): 547-556



Neuraminidase activity could be demonstrated in highly concentrated preparations of human parainfluenza 1 virus, strain C35 (HA2 virus). Among the substrates used, the most suitable are N-acetyl neuramin lactose and fetuin. Mucin type I and type II were not hydrolyzed. The neuraminidase exhibited some characteristics similar to those of the other paramyxoviruses (Sendai, NDV [Newcastle disease virus], mumps, human parainfluenza 2 virus): the optimum pH ranged between 5-5.4 and the Km value was 5 .times. 10-3 M when tested with N-acetyl neuramin lactose. Its optimum activity was between 37.degree.-40.degree. C and it was thermolabile, the enzyme activity being reduced to 50% in 5 min at 45.degree. C and entirely destroyed at 50 C in the same period. The thermal inactivation constants of neuraminidase and hemagglutinin and the temperature which inactivated 50% of both these activities were very similar to those already shown for NDV. Hemagglutinin and neuraminidase activities were rapidly destroyed by ionic detergents, but not by non-ionic detergents.

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