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The nucleotide sequence and derived amino acid sequence of complementary dna coding for mouse carbonic anhydrase ii ec 4.2.1.10


The nucleotide sequence and derived amino acid sequence of complementary dna coding for mouse carbonic anhydrase ii ec 4.2.1.10



Gene 25(2-3): 325-332



ISSN/ISBN: 0378-1119

The nucleotide sequence of a clone containing mouse carbonic anhydrase (CA) c[complementary]DNA in pBR322 was determined. The cloned cDNA contains all of the coding region except for nucleotides specifying the first 8 amino acids, and all of the 3' noncoding region, which consists of 700 nucleotides. A cDNA clone was identified which contains an additional 54 bp [base pair] at the 5' end, so that the complete amino acid sequence of mouse CA could be deduced. This sequence showed a 73-81% homology with other mammalian CA form II isozymes, 56-63% with form I isozymes, and 52-56% with form III isozymes. By examination of the amino acids which are unique and invariant for each isozyme, the mouse amino acid sequence was found to contain 16 of the 23 residues that are unique and invariant to mammalian CA form II isozymes, but only one or no residue for forms I and III, respectively.

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Accession: 006725632

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