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The oncogenic potential of 3 different 7 12 di methyl benz a anthracene transformed c 3h 10t 1 2 cell clones at various passages and the importance of the mode of immuno suppression


, : The oncogenic potential of 3 different 7 12 di methyl benz a anthracene transformed c 3h 10t 1 2 cell clones at various passages and the importance of the mode of immuno suppression. European Journal of Cancer 15(4): 515-526

The oncogenic potential of C3H/10T1/2 [mouse] cells which was transformed in vitro with 7,12-dimethylbenz(a)anthracene is reported. The ability of the cells to grow as malignant tumors in syngeneic immunosuppressed mice was used as parameter for oncogenic potential. Cells of types I, II and III were assayed at several dosage levels, i.e., 104 or 106 cells per inoculum, with or without immunosuppression by antithymocyte serum globulin fraction. The studies were performed in several strains of host animals, i.e., male and female syngeneic C3H mice, C3H mice and nude, athymic female mice. Morphological transformation preceded oncogeneic transformation and type I cells could not be established as tumors. Type II and type III cells developed oncogeneic potential only after several passages in culture. Oncogeneic potential was pronounced in the type III cells, and less strongly expressed in type II cells. Also tested were different methods of immunosuppression of the animal against the expression of the oncogeneic potential of DMBA transformed C3H/10T1/2 cells from type II and III clones. Immunosuppression by antithymocyte serum globulin fraction was an effective method of preparing the syngeneic host so that cells with a low oncogeneic potential would grow as tumors, whereas total body irradiation was not effective. For cells with a high oncogeneic potential both ways of immunosuppression were sufficient. Admixing lethally irradiated cells in the cell inoculum slightly enhanced the tumor development from cells with low oncogeneic potential and such addition was clearly effective for cells with a higher oncogeneic potential, both for the antibody-treated and for the irradiated series. The study stresses the importance of immunosuppression by antithymocyte globulins for detecting in vitro transformed weakly oncogeneic cells.

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