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The optimal conditions of colony stimulating factor generation by endo toxic lipo poly saccharide and its ps derivative in mice


, : The optimal conditions of colony stimulating factor generation by endo toxic lipo poly saccharide and its ps derivative in mice. Journal of Immunopharmacology 5(1-2): 93-106

Not only the endotoxic lipopolysaccharide (LPS) preparations, but a nontoxic, lipid-free, non-mitogenic hydrolytic breakdown product of it (called PS) is also capable of inducing colony stimulating factor (CSF) release. Due to difficulties in reproducing the above findings it became necessary to study the optimal conditions to obtain CSF active PS preparations. It was found that the CSF generating component of the highly heterogeneous PS mixture is sensitive to acidic hydrolyses, but it is less sensitive than the toxic site in the lipid moiety of the LPS. Carefully controlled optimal hydrolytic conditions give PS preparations which have < 1% residual endotoxicity but maintain 40-80% of the original CSF generating capacity. Prolonged hydrolysis will destroy this activity too. Optimal dose of LPS and PS for CSF induction in mice differed widely. For LPS the optimal dose is 25 .mu.g, injecting more gave a much reduced or nondetectable CSF level. Optimal dose for PS was 160 .mu.g, and this generated a significantly higher CSF level than 25 .mu.g LPS. At concentrations below 25 .mu.g, LPS was clearly more active than PS. The CSF level reached its peak at 3-4 h after the other LPS or PS injection. The i.v. route was sometimes but not always more effective than the i.p. route.

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