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The organization and repair of dna in the mammalian chromosome part 2 a gradient shape induced speed dependence affecting dna larger than t 4 dna


, : The organization and repair of dna in the mammalian chromosome part 2 a gradient shape induced speed dependence affecting dna larger than t 4 dna. Biopolymers 16(5): 1083-1092

The addition of a lytic layer to a preformed linear sucrose gradient induced a temporary (up to half a day) initial gradient of considerable steepness which retards the sedimentation of large (> [phage] T4) DNA. Centrifugation at sufficiently slow angular velocities permits the temporary initial gradient to disappear and therefore the sedimentation distance increased, yielding a rotor speed dependent effect on sedimentation distance. Gradients which are free of this effect were described and permitted mouse leukemia [L5178y] cell DNA to sediment independently of rotor speed (5-30 krpm).

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