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The organization and repair of dna in the mammalian chromosome part 2 a gradient shape induced speed dependence affecting dna larger than t 4 dna


, : The organization and repair of dna in the mammalian chromosome part 2 a gradient shape induced speed dependence affecting dna larger than t 4 dna. Biopolymers 16(5): 1083-1092

The addition of a lytic layer to a preformed linear sucrose gradient induced a temporary (up to half a day) initial gradient of considerable steepness which retards the sedimentation of large (> [phage] T4) DNA. Centrifugation at sufficiently slow angular velocities permits the temporary initial gradient to disappear and therefore the sedimentation distance increased, yielding a rotor speed dependent effect on sedimentation distance. Gradients which are free of this effect were described and permitted mouse leukemia [L5178y] cell DNA to sediment independently of rotor speed (5-30 krpm).

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Related references

Lange, C.S.; Martin, P.; Ferguson, P.; Resnick, M.A., 1977: The organization and repair of DNA in the mammalian chromosome. II. A gradient-shape-induced speed dependence affecting DNAs larger than T4 DNA. Biopolymers 16(5): 1083-1092

Lange, C.S.; Liberman, D.F.; Clark, R.W.; Ferguson, P.; Sheck, L.E., 1977: The organization and repair of dna in the mammalian chromosome part 3 determination of the molecular weight of a mammalian native dna. The necessary conditions for a unique solution of the sedimentation vs. DNA MW equations are considered and applied to the native DNA of the L5178Y mouse leukemia cell. Previously described sedimentation anomalies were given to demonstrate that su...

Lange, C.S.; Liberman, D.F.; Clark, R.W.; Ferguson, P.; Sheck, L.E., 1977: The organization and repair of DNA in the mammalian chromosome. III. Determination of the molecular weight of a mammalian native DNA. Biopolymers 16(5): 1093-1014

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