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The organization of histones and dna in chromatin evidence for an arginine rich kernel


, : The organization of histones and dna in chromatin evidence for an arginine rich kernel. Cell 8(3): 333-347

The roles of various histones in the organization of the DNA of the nucleosome were examined, using staphylococcal nuclease as a probe of DNA conformation from duck erythrocyte nuclei. When this enzyme attacks chromatin, a series of fragments evenly spaced at 10 base pair intervals is generated, reflecting the histone-DNA interactions within the nucleosome structure. To determine what contribution the various histones make to DNA organization, the staphylococcal nuclease digestion patterns of DNA-purified histone complexes were studied. Virtually all possible combinations of homogeneous histones were reconstituted onto DNA. Exhaustive digestion of a complex containing the 4 histones H2A, H2B, H3 and H4 yields a DNA fragment pattern very similar to that of whole chromatin. The only combinations of histones capable of inducing chromatin-like DNA organization are H2A/H2B/H4 and those mixtures containing both H3 and H4. From an examination of the kinetics of digestion of H3/H4 reconstitutes, it was concluded that although the other histones have a role in DNA organization within the nucleosome, the arginine-rich histone pair, H3/H4, can organize DNA segments the length of the nucleosome core in the absence of all other histones.

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Related references

Camerini-Otero, R.D.; Sollner-Webb, B.; Felsenfeld, G., 1976: The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel. We have examined the role played by various histones in the organization of the DNA of the nucleosome, using staphylococcal nuclease as a probe of DNA conformation. When this enzyme attacks chromatin, a series of fragments evenly spaced at 10 base...

Krause, M.O.; Yoo, B.Y.; Macbeath, L., 1974: Histones from exponential and stationary L-cells. Evidence for differential binding of lysine-rich and arginine-rich fractions in chromatin. Archives of Biochemistry and Biophysics 164(1): 172-178

Clark, R.J.; Felsenfeld, G., 1972: Association of arginine-rich histones with G-C-rich regions of DNA in chromatin. Nature: New Biology 240(103): 226-229

Vandegrift, V.; Serra, M.; Moore, D.S.; Wagner, T.E., 1974: The role of the arginine-rich histones in the maintenance of DNA conformation in chromatin. Biochemistry 13(25): 5087-5092

Olson M.O.J.; Sugano N.; Yeoman L.C.; Johnson B.R.; Jordan J.; Taylor C.W.; Starbuck W.C.; Busch H., 1972: Homology of the amino terminal sequences of the arginine lysine rich and glycine arginine rich calf thymus histones. Physiological Chemistry & Physics 4(1): 10-16

Puigdomenech P.; Cabre O.; Palau J.; Bradbury E.M.; Crane Robinson C., 1975: Studies on the role and mode of operation of the very lysine rich histones in eukaryote chromatin the conformation of 1 histones from marine invertebrate sperm. European Journal of Biochemistry 59(1): 237-243

Puigdoménech, P.; Cabré, O.; Palau, J.; Bradbury, E.M.; Crane-Robinson, C., 1975: Studies on the role and mode of operation of the very-lysine-rich histones in eukaryote chromatin. The conformation of phi1 histones from marine invertebrate sperm. Proton magnetic resonance, circular dichroism and infrared spectroscopy are used to investigate the secondary and tertiary structure of three very lysine-rich histones from marine invertebrate sperm. At high ionic strength both Arbacia lixula and...

Weihe, A.; von Mickwitz, C.U.; Grade, K.; Lindigkeit, R., 1978: Complexes of DNA with arginine-rich and slightly lysine-rich histones. Transcription and electron microscopy. Calf thymus DNA was reconstituted with arginine-rich histones H3 and H4 and slightly lysine-rich histones H2A and H2B respectively. Complexes containing histones H3 and H4 exhibit nucleosome-like structures when examined in the electron microscope...

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Pinevich A.V.; Zhdan Pushkina S.M., 1972: Study of sorption specificity and interaction with dna of arginine rich and lysine rich histones the mechanism of anti bacterial action. Vestnik Leningradskogo Universiteta Seriya Biologii 27(2): 109-113