EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The organization of histones and dna in chromatin evidence for an arginine rich kernel


, : The organization of histones and dna in chromatin evidence for an arginine rich kernel. Cell 8(3): 333-347

The roles of various histones in the organization of the DNA of the nucleosome were examined, using staphylococcal nuclease as a probe of DNA conformation from duck erythrocyte nuclei. When this enzyme attacks chromatin, a series of fragments evenly spaced at 10 base pair intervals is generated, reflecting the histone-DNA interactions within the nucleosome structure. To determine what contribution the various histones make to DNA organization, the staphylococcal nuclease digestion patterns of DNA-purified histone complexes were studied. Virtually all possible combinations of homogeneous histones were reconstituted onto DNA. Exhaustive digestion of a complex containing the 4 histones H2A, H2B, H3 and H4 yields a DNA fragment pattern very similar to that of whole chromatin. The only combinations of histones capable of inducing chromatin-like DNA organization are H2A/H2B/H4 and those mixtures containing both H3 and H4. From an examination of the kinetics of digestion of H3/H4 reconstitutes, it was concluded that although the other histones have a role in DNA organization within the nucleosome, the arginine-rich histone pair, H3/H4, can organize DNA segments the length of the nucleosome core in the absence of all other histones.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006728976

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Freire, J.C.; Souza, J.J.; Lopes, A.S.; Bahia, V.G.; Bahia, F.G.F.T.C., 1976: Available water in two soils of the Lavras region. Tables give the water content of different soil layers (0-15 to 70-100 cm) at different tensions (0.1 to 20 atm) in a dark red latosol and a red-yellow podzolic soil under natural vegetation, eucalyptus forest, pasture and arable crops. Available...

Li, Y.; Li, Z.; Chang, G.; Wang, M.; Wu, R.; Wang, S.; Yao, C., 2015: Effect of structured home-based exercise on walking ability in patients with peripheral arterial disease: a meta-analysis. The present study is designed to investigate the effect of structured home-based exercise (SHE) programs on maximal walking time (MWT), pain-free walking time (PFWT), and self-reported walking ability in patients with peripheral arterial disease (...

Elifantz, H.; Malmstrom, R.R.; Cottrell, M.T.; Kirchman, D.L., 2005: Assimilation of polysaccharides and glucose by major bacterial groups in the Delaware Estuary. The contribution of major bacterial groups to the assimilation of extracellular polymeric substances (EPS) and glucose in the Delaware Estuary was assessed using microautoradiography and fluorescence in situ hybridization. Bacterial groups contrib...

Jagdeo, J.; Kroshinsky, D., 2011: A case of post-herpetic itch resolved with gabapentin. Herpes zoster is a skin disease encountered commonly in dermatologic practice. While post-herpetic neuralgia (PHN) is a familiar side effect of this condition, it is not the only neuropathic symptom that can result. Post-herpetic itch (PHI) is les...

Saynor, M.; Hill, D.S., 1977: Chemical control of onion fly delia antiqua. In 9 trials done in 1972-74 seed treatments [onions] reduced attacks of onion fly larvae very effectively and most of the organophosphorus formulations gave protection up to harvest, 12-17 wk after sowing. Pirimiphos-ethyl and isofenphos each at 1...

Kagan, Y.R.; Anishchenko, I.P.; Sergeeva, I.Y.; Dremkova, B.I., 1983: Determination of total count of microorganisms during manufacture of Sovetskii cheese. Because cheesemaking temp. generally favour development of microflora with optimal growth temp. below the 37 degrees C incubation temp. recommended for total bacterial counts in regulations for USSR dairy industry factories, total bacterial counts...

Makolkin, V.I.; Bazhanov, N.N.; Men'shikova, I.V.; Petukhova, N.V., 1994: Variants of clinical course of myocarditis in systemic lupus erythematosus. Kardiologiya 34(1-2): 79-82

Wagner, C.; Mohme, H.; Krömer-Olbrisch, T.; Stadler, R.; Goerdt, S.; Kurzen, H., 2009: Langerhans cell histiocytosis: treatment failure with imatinib. Archives of Dermatology 145(8): 949-950

Anonymous, 1997: Honorary member. Canadian Journal of Plant Pathology 3

Fassati, P.; Motejlová, A.; Fassati, M.; Horejsí, J.; Spízek, J.; Oktábcová, M.; Schön, E.; Kopriva, P., 1990: Hormonal contraception and levels of selected serum proteins. 116 women 21-36 years old, who had been taking oral contraceptives (OC) for at least 1 and 1/2 years and, at the most, for 4 years, were studied. The subjects underwent detailed clinical, biochemical, and immunological tests using samples of thei...