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The preservation of muellerian inhibiting substance during long term freezing of testicular fragments






Cryobiology 14(5): 534-542

The preservation of muellerian inhibiting substance during long term freezing of testicular fragments

Muellerian inhibiting substance [MIS], a fetal testicular hormone found in most mammalian species, causes regression in the male of the Muellerian duct, the anlagen of the fallopian tube, uterus and upper vagina. Limitations to study of this substance in the past have been posed by its short period of production and by its localized and specific action. It was possible to store testicular fragments [fetal and newborn rats and calves] that continue to demonstrate detectable MIS activity for up to 5 mo. by using techniques of slow freezing which approximate 1.degree. C/min, cryoprotective additives, storage in liquid N and rapid thawing. These fragments then can be pooled for biochemical and endocrinological studies. In addition, unknown fragments can be transported long distances for assay of MIS.


Accession: 006740016

PMID: 561675



Related references

Donahoe, P.K.; Ito, Y.; Hendren, W.H., 1977: The preservation of Mullerian inhibiting substance during long-term freezing of testicular fragments. Muellerian inhibiting substance [MIS], a fetal testicular hormone found in most mammalian species, causes regression in the male of the Muellerian duct, the anlagen of the fallopian tube, uterus and upper vagina. Limitations to study of this subst...

Matzuk, M.M.; Finegold, M.J.; Mishina, Y.; Bradley, A.; Behringer, R.R., 1995: Synergistic effects of inhibins and Muellerian-inhibiting substance on testicular tumorigenesis. Members of the transforming growth factor-beta (TGF-beta) superfamily regulate diverse physiological processes in multiple tissues. In particular, important roles for the inhibins and Mullerian-inhibiting substance (MIS) have been demonstrated in...

Hutson J.M.; Shaw G.; O.W.S.; Short R.V.; Renfree M.B., 1988: Muellerian inhibiting substance production and testicular migration and descent in the pouch young of a marsupial. The ontogeny of Muellerian inhibiting substance (MIS) production by the developing testis of an Australian marsupial, the tammar wallaby (Macropus eugenii), was determined during pouch life using an organ-culture bioassay of mouse fetal urogenital...

Taketo, T.; Saeed, J.; Nishioka, Y.; Donahoe, P.K., 1991: Delay of testicular differentiation in the b6.y d o m ovotestis demonstrated by immunocytochemical staining for muellerian inhibiting substance. It has been found that when the Y chromosome from Mus musculus domesticus (YDOM) is placed onto the C57BL/6J (B6) mouse background, the XY progeny (B6.YDOM) develop ovaries or ovotestes but not normal testes during fetal life. We examined the onto...

Taketo T., 1991: Production of muellerian inhibiting substance mis and sulfated glycoprotein 2 sgp 2 associated with testicular differentiation in the xx mouse gonadal graft. Robaire, B (Ed ) Annals Of The New York Academy Of Sciences, Vol 637 The Male Germ Cell: Spermatogonium to Fertilization; Xith North American Testis Workshop, Montreal, Quebec, Canada, April 24-27, Xi+510p New York Academy Of Sciences: New York, New York, Usa Illus 74-89

Teng C.S.; Wang J.J.; Teng J.I.N., 1987: Purification of chicken testicular muellerian inhibiting substance by ion exchange and high performance liquid chromatography. A highly purified protein molecule was obtained from the secretory proteins of 8-week-old chicken testes using ion-exchange column chromatographic procedures, including DEAE Bio-Gel A, CM Bio-Gel A, wheat germ lectin columns, and high-performance...

Taketo, T.; Saeed, J.; Manganaro, T.; Takahashi, M.; Donahoe, P.K., 1993: Muellerian inhibiting substance production associated with loss of oocytes and testicular differentiation in the transplanted mouse XX gonadal primordium. The mouse XX gonadal primordium develops seminiferous-like tubules after transplantation into the renal subcapsular site of the adult male or female mouse. We examined the ontogeny of Sertoli cell differentiation in XX gonadal grafts by immunocyto...

Price, J.M.; Donahoe, P.K.; Ito, Y.; Hendren, W.H.I.i, 1977: Programmed cell death in the muellerian duct induced by muellerian inhibiting substance. The importance of a secretory product of the fetal testis, Muellerian inhibiting substance [MIS] in determining the fate of the Muellerian duct in the developing male fetus was well documented. The mechanism of action of MIS in the male rat fetus...

Teng C.S., 1983: The correlation of glycolization in muellerian inhibiting substance and its activity for muellerian duct regression. Journal Of Cell Biologypart 2: 38a

Price J.M., 1979: Involution of the muellerian duct under the influence of the muellerian inhibiting substance in an organ culture assay. Anatomical Record 193(3): 657-658