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The preservation of muellerian inhibiting substance during long term freezing of testicular fragments


, : The preservation of muellerian inhibiting substance during long term freezing of testicular fragments. Cryobiology 14(5): 534-542

Muellerian inhibiting substance [MIS], a fetal testicular hormone found in most mammalian species, causes regression in the male of the Muellerian duct, the anlagen of the fallopian tube, uterus and upper vagina. Limitations to study of this substance in the past have been posed by its short period of production and by its localized and specific action. It was possible to store testicular fragments [fetal and newborn rats and calves] that continue to demonstrate detectable MIS activity for up to 5 mo. by using techniques of slow freezing which approximate 1.degree. C/min, cryoprotective additives, storage in liquid N and rapid thawing. These fragments then can be pooled for biochemical and endocrinological studies. In addition, unknown fragments can be transported long distances for assay of MIS.

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