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The preservation of salt marsh algae by controlled liquid nitrogen freezing


Cryobiology 15(5): 563-568
The preservation of salt marsh algae by controlled liquid nitrogen freezing
Five species of benthic marine algae were preserved by controlled liquid N freezing and storage over periods extending to 1 yr. Only a small percent of the algae survived without cryoprotectant. Nanochloris adamsii was an exception; 67% survived after 12 mo. of storage. Nitzschia acicularis was the best preserved with 5 glycerol as a cryoprotectant. Dimethylsulfoxide was a better cryoprotectant for N. adamsii and Dunaliella quartolecta. Reducing normal brackish salinity (28.permill.) of the culture medium to 1/2 (14.permill.) increased the survival percentages for N. acicularis, Cylindrotheca closterium and Phaeodactylum tricornutum. The morphology and physiology of the species tested were unchanged by long storage time in liquid N.

Accession: 006740024

PMID: 363360

DOI: 10.1016/0011-2240(78)90079-2

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