EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The preservation of soluble material on the surface and in cavities of the pollen wall of campanulaceae and pentaphragmataceae


Micron 12(1): 47-64
The preservation of soluble material on the surface and in cavities of the pollen wall of campanulaceae and pentaphragmataceae
During conventional preparation for transmission electron microscopy a considerable part of the soluble material on the surface and in cavities of the pollen wall and in the anther loculus between the tapetum and the pollen grains is extracted. To improve rehydration of dried pollen grains and stabilize surface material of the pollen wall, different compound fixatives and a modified dehydration technique was applied. Structures are retained by the specialized treatment which are not observed when buffered glutaraldehyde alone is used. Intine-held vesicles and a fibrillar network in gaps and spaces of the endexine stain positively for protein.


Accession: 006740026

DOI: 10.1016/0047-7206(81)90019-4



Related references

Pollen morphology and taxonomic position of the genus Pentaphragma Wall. (Pentaphragmataceae). The use of compound fixatives. Grana (3) 141-147, 1978

On pollen of Campanulaceae and related families with special reference to the surface ultrastructure. I. Campanulaceae Subfam. Campanuloidae. Botaniska Notiser 12(1): 73-101, 1975

On pollen of campanulaceae and related families with special reference to the surface ultrastructure part 1 campanulaceae subfamily campanuloideae. Botaniska Notiser 128(1): 73-101, 1975

Palynomorphology of the families campanulaceae sphenocleaceae and pentaphragmataceae. Botanicheskii Zhurnal (St Petersburg) 71(8): 1003-1010, 1986

On pollen of campanulaceae and related families with special reference to the surface ultrastructure part 2 campanulaceae subfamily cyphioidae and subfamilies lobelioidae goodeniaceae sphenocleaceae. Botaniska Notiser 128(1): 102-118, 1975

On pollen of Campanulaceae and related families with special reference to the surface ultrastructure. II. Campanulaceae Subfam. Cyphioidae and Subfam. Lobelioidae; Goodeniaceae; Sphenocleaceae. Botaniska Notiser 12(1): 102-118, 1975

The use of cetyl pyridinium chloride to preserve water soluble surface material in pollen for electron microscopy. Micron 14(1): 29-32, 1983

Techniques to preserve soluble surface components in birch pollen wall: a scanning and transmission electron microscopic study. Journal of Histochemistry and Cytochemistry 37(7): 981-987, 1989

The use of cetylpyridinium chloride to preserve water-soluble surface material in pollen for electron microscopy Corylus avellana, Pinus mugo. Micron 4(1): 29-32, 1983

Pollen surface morphology in north american campanula triodanis and heterocodon campanulaceae. American Journal of Botany 74(5): 746-747, 1987