EurekaMag.com logo
+ Translate

The preservation of the mechanical properties of venous allo grafts by freezing


, : The preservation of the mechanical properties of venous allo grafts by freezing. Journal of Surgical Research 27(4): 239-243

Stored venous allografts are potential alternatives to autogenous materials when the latter are unavailable for arterial replacement. Among the criteria necessary for utilization would be the preservation of hemodynamically favorable elastic properties. The effects of frozen storage on venous compliance (percentage volume change/mm Hg) and elastic modulus (dyn/cm2 .times. 106) of 40 canine jugular vein segments was studied. The veins were allotted to 2 experimental groups. Five from each group acted as controls. Vein segments [15] were frozen immediately with liquid N2(-196.degree. C) and stored at -70.degree. C (unprotected). Another 15 were pretreated with 15% DMSO [dimethylsulfoxide] and then frozen (DMSO). The veins were thawed by immersion in Hanks' balanced salt solution (37.degree. C) after either 1 or 24 h, or 1 wk. Compliance and elastic modulus were calculated from the volumetric responses to pressure of the vein segments mounted in a special saline-filled cylinder. There was a modest but statistically insignificant increase in compliance from initial control values of 1.9 .+-. 0.68 (unprotected) and 1.3 .+-. 0.44 (DMSO-treated) to 2.16 .+-. 0.29 and 2.04 .+-. 0.70, respectively, at 1 wk. The elastic moduli of the control groups (16.3 .+-. 4.5, unprotected; 22.6 .+-. 4.5, DMSO) were also indistinguishable from the frozen groups (13.5 .+-. 2.3, unprotected; 15.4 .+-. 3.9, DMSO). Short-term freezing preserves the important functional elastic properties of veins and this preservation is independent of the use of DMSO.

(PDF 0-2 workdays service)

Accession: 006740032

PMID: 480947

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Shah K.H.; Bitter Suermann H.; Save Soderbergh J., 1980: Morphological findings in duct ligated pancreas grafts in the rat an analysis of iso grafts allo grafts and long standing allo grafts in hosts conditioned by previous spleen allo grafts. The morphological findings of duct-ligated pancreas grafts in streptozotocin-induced diabetic hosts were studied using inbred AGUS and WAG rats with a major histocompatibility complex difference. AGUS to AGUS pancreas isografts survived indefinite...

Laforest P.; Willinger R., 1988: Mechanical properties of deep freezing bone grafts. Biomaterials Artificial Cells & Artificial Organs 16(4): 868

Triantafyllou N.; Karatzas P., 1975: Effects of sterilizing doses of ionizing radiation on the mechanical properties of various connective tissue allo grafts. Krippner, Monica (Ed ) Radiosterilization Of Medical Products 1974 Proceedings Of The Symposium on Ionizing Radiation For Sterilization Of Medical Products And Biological Tissues Bombay, India, December 9-13, 1974 539p Illus International Atomic Energy Agency: Vienna, Austria (Dist in U S A By Unipub: New York, N Y , U S A ) Isbn 92-0-010475-4 215-223

Pechan, J.; Stvrtina, S.; Bolf, J.; Schnorrer, M., 1986: Apparatus for measuring the mechanical properties of venous grafts. Bratislavske Lekarske Listy 86(4): 363-369

Marshall V.C.; Ross H.; Scott D.F.; Mcinnes S.; Atkins R.C., 1977: Preservation of cadaveric renal allo grafts. Kidney International 12(1): 84

Bettin, D.; Polster, Jürgen.; Rullkötter, V.; von Versen, Rüdiger.; Fuchs, S., 2003: Good preservation of initial mechanical properties in lipid-extracted, disinfected, freeze-dried sheep patellar tendon grafts. Patellar tendon allografts can be used for anterior cruciate reconstruction to avoid morbidity of autografts on the donor side. Secondary processing of allografts is important for reducing immunological reactions, bacterial contamination and impro...

Anonymous, 1981: Differential acceptance of skin allo grafts on rats having primary vs transferred tolerance to cardiac allo grafts

Warren R.P.; Lofgreen J.S.; Steinmuller D., 1973: Differential survival of heart allo grafts and skin allo grafts in inbred rats. RAPAPORT, FELIX T , HANS BALNER AND SAMUEL L KOUNTZ (ED ) TRANSPLANTATION TODAY, VOL II PROCEEDINGS OF THE FOURTH INTERNATIONAL CONGRESS OF THE TRANSPLANTATION SOCIETY SAN FRANCISCO, CALIF , U S A, SEPT 24-29, 1972 1016P ILLUS GRUNE and STRATTON: NEW YORK, N Y , U S A ; LONDON, ENGLAND 717-719

Morris P.J., 1984: Islet allo grafts in rats made tolerant of renal allo grafts by whole blood transfusion compared to islet allo grafts after blood transfusion alone. Transplantation Proceedings: 863-864

Murphy J.J., 1970: Histo compatability testing in human renal allo grafts part 1 evidence for a strong and a weak hl a sub locus in recipients of allo grafts from living related donors. Cleveland Clinic Quarterly: 13-21