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The pressure dependence of the spin equilibrium in camphor bound iron iii cytochrome p 450


, : The pressure dependence of the spin equilibrium in camphor bound iron iii cytochrome p 450. European Journal of Biochemistry 124(2): 311-316

The spin equilibrium of camphor-bound ferric [Pseudomonas putida] cytochrome P-450 was measured between 1-1000 bar (105-108 Pa [pascal]). Increasing pressure shifts the absorption spectrum from the high-spin form at 392 nm to the low-spin form at 417 nm. The molar volume change for the spin states .DELTA.V = - RT.delta.ln Ke/.delta.P and the equilibrium coefficient Ke = [high spin]/[low spin] depend on the solvent conditions. At pH 5.6 the equilibrium coefficient at 1 bar, K1 = 0.5 and .DELTA.V = 31 cm3/mol. A sample with 10 mM KCl at pH 7 has K1 = 7.0 and .DELTA.V = 52 cm3/mol. Solvent changes producing a larger K1 also result in a larger .DELTA.V which ranged over 16-74 cm3/mol. The correlation can be approximated as .DELTA.V = 36 + 18 log K1, which implies that there is a pressure, 3000 bar, for camphor-bound ferric cytochrome P-450 at 4.degree. C, at which the changes in .DELTA.V are compensated by the other thermodynamic parameters leaving Ke independent of the solvent conditions. Although the protein is not stable above 1000 bar for most sample conditions, the extrapolated log Ke vs. pressure curves for all sample conditions intersect near 3000 bar. Camphor-bound cytochrome P-450 appears to be a rather flexible protein, having a low denaturing pressure, a large volume change, and a high sensitivity to the protein environment.

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