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The presynaptic grid a new approach


Brain Research 184(1): 23-40
The presynaptic grid a new approach
A new EM facility is described which enables systematic visualization of E-PTA[ethanolic phosphotungstic acid]-stained presynaptic grids in full en face position. This EM-facility is used to analyze the size and the number of dense projections of synaptic grids in different brain areas of the rabbit. The view that dense projections form an intrinsic part of all central synapses and are organized in a hexagonal or triangular pattern is supported. The observation of annulate and horseshoe-shaped synaptic grids agrees with previous observations on synaptic connections with subsynaptic plate perforations. A non-normal frequency distribution of dense projections per synaptic grid with distinct peaks is suggestive of the existence of distinct size classes of synaptic contacts. Analyses of the frequency distribution of dense projections in different areas and at different levels below the pial surface in adult animals point to a specificity of the distinct size classes related to the afferent origin or the target cell on which they are projecting. Investigation or grid size and number of dense projections during a period of rapid synaptogenesis reveals that newly formed synapses also have a specific size. The complementarity of dense projections and vesicle attachment sites implicit in the model of Akert et al (1972) was used to calculate the number of vesicle attachment sites per sq. .mu.m because of the density of projections per grid. The agreement between these values and those of Akert and Peper (1975), based on the analyses of vesicle attachment sites in freeze-etch specimen, is striking. The possible implications of these observations in relation to synaptic efficacy and neuronal microcircuitry are discussed.

Accession: 006740097

PMID: 7357422

DOI: 10.1016/0006-8993(80)90585-5

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