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The prickly lettuce agglutinin 1. isolation from leaves of the prickly lettuce plant lactuca scariola


, : The prickly lettuce agglutinin 1. isolation from leaves of the prickly lettuce plant lactuca scariola. International Archives of Allergy and Applied Immunology 61(1): 32-39

An agglutinin was isolated from leaves of the prickly lettuce plant (L. scariole). The prickly lettuce agglutinin (PLA) agglutinated erythrocytes of the mouse and rat, and bound to, but did not agglutinate erythrocytes of other species [rabbit, guinea pig, chicken and human]. PLA could be precipitated from crude plant extracts by cold acetone and ammonium sulfate treatment, was sensitive to proteolytic enzyme activity, and contained carbohydrate residues, suggesting that it is a glycoprotein. The agglutinin has a MW of approximately 84,000 as determined by SDS-PAGE [sodium dodecyl sulfate-polyacrylamide gel electrophoresis] studies. The receptor for PLA has not been elucidated but may involve, in part, sialic acid residues.

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