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The primary investigation of epidemiology on the buzura suppressaria nuclear polyhedrosis virus npv


, : The primary investigation of epidemiology on the buzura suppressaria nuclear polyhedrosis virus npv. Virologica Sinica 1(4): 87-92

Buzura suppressaria NPV has been the most widely used virus by artificial dissemination of NPV in the tea plantation with trial conducted in Hubei, Guangdong, Jiangxi go on[China]. In puqi, in 1979, 1981, 1983, dosages of 2 .times. 108 PIB/ml, were tested and Tea garden were sprayed to 750 liter/ha. At 10 days postspray these dosages gave 97.8% mortality, with 12% mortality, respectively, with 15% mortality on untreated. Anyway, BSNPV had maintained in a viable state on foliage for more days or several years. The polyhedra were observed at the egg surface by scanning electron microscope.

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Related references

Gan, Y-k., 1981: Studies on the nuclear polyhedrosis virus of Buzura suppressaria. In 1977, a nuclear polyhedrosis virus of the tea pest Buzura suppressaria (Gn.) was isolated from dead larvae collected in Hunan, China. The properties of the virus are described. LC50s for 4th- and 5th-instar larvae following ingestion of infecte...

H.Z.H.; Xie T E., 1991: Plaque assay of buzura suppressaria nuclear polyhedrosis virus. The plaque assay was carried out of Buzura suppressaria nuclear polyhedrosis virus (BsNPV) using cell line Bs-484. Results showed that the sensitivity of this plaque assay method was similar to that of TCID50 method.

Liu, M.; Hu, Z.; Liang, B.; Jin, F.; Li, M.; Xie, T., 1993: Physical mapping of buzura suppressaria nuclear polyhedrosis virus genome. A physical map of the genome of buzura suppressaria singly-enveloped nuclear polyhedrosis virus (BsSNPV) was determined using six restriction endonucleases (RE), singly or in pairs, and Southern hybridizations of cloned or gel-purified DNA fragmen...

Meng X.; Liu N.; Ding Q., 1988: Homology of buzura suppressaria nuclear polyhedrosis virus dna with dna of other insect baculoviruses by dot hybridization. BsNPV-DNA probe was prepared by nick translation. Dot hybridization was carried out on the nitricellulose membrane with six other insect baculoviruses, Buzura thibetaria NPV (BtNPV), Ectropis obliqua hypulina NPV (EoNPV), Chihuo zao Yang NPV (CzNP...

Liu N C.; Meng X L., 1988: Analysis of the structural polypeptides of nuclear polyhedrosis virus from buzura suppressaria guenee. The structural polypeptides of Buzura suppressaria Guenee nuclear polyhedrosis virus was studied by vertical slab polyacrylamide gel electrophoresis. The results revealed that the polyhedrin.sbd.the only major polypeptide with molecular weight of...

Xu, X.; Zhang, C.; Xie, T.'en, 1995: Studies on infection of Buzura suppressaria nuclear polyhedrosis virus in Bs484 cells. The part of effects and characters of infection with Buzura suppressaria nuclear polyhedrosis virus (BsNPV) for Bs484 cells in the different media and cultural time were reported in this paper. The results showed that the types of media and the ti...

Liu M F.; H.Z.H.; Jin F.; L.M.J.; Xie T E., 1992: Localization and cloning of the polyhedrin gene of buzura suppressaria nuclear polyhedrosis virus. The polyhedrin gene of tung-oil geometrid, Buzura suppressaria, nuclear polyhedrosis virus (BsNPV) was localized on BamHI-B, BglI-A, BglII-F, EcoRI-P, HindIII-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fragments under low stringence (40% formamid...

Zhu, Q-q.; Liu Jun.; Liu Yulan., 1982: The detection of nuclear polyhedrosis virus in Buzura suppressaria and Bombyx mori by using the method of ELISA. Acta Entomologica Sinica, 252: 223-226

Hu, Z.H.; Liu, M.F.; Jin, F.; Wang, Z.X.; Liu, X.Y.; Li, M.J.; Liang, B.F.; Xie, T.E., 1993: Nucleotide sequence of the Buzura suppressaria single nucleocapsid nuclear polyhedrosis virus polyhedrin gene. A portion of the genome of the Buzura suppressaria (Lepidoptera) single nucleocapsid nuclear polyhedrosis virus (BsSNPV) containing the polyhedrin gene was sequenced. An open reading frame of 738 nucleotides encoded a protein of 246 amino acids an...

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