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The primary isolation of Leishmania donovani from Iraq on different culture media

, : The primary isolation of Leishmania donovani from Iraq on different culture media. Annals of Tropical Medicine and Parasitology 73(4): 345-347

Leishmania donovani was isolated by culturing bone marrow aspirates from kala-azar patients, on three types of culture media. The isolation was successful in 88% of the cases. The effects of pH, D-glucose, L-proline and antibiotic concentration in the isolation were investigated. The semi-solid medium used (pH 7.4) with L-proline, was better than the modified NNN media for the primary isolation.

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Accession: 006740739

PMID: 496486

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