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The primary processes of photosystem ii in purified guard cell protoplasts and mesophyll cell protoplasts from commelina communis

, : The primary processes of photosystem ii in purified guard cell protoplasts and mesophyll cell protoplasts from commelina communis. Planta (Heidelberg) 159(6): 554-560

Guard-cell protoplasts were isolated by enzymic digestion of the epidermis peeled from the abaxial surface of leaves from C. communis L. The protoplasts were separated from mesophyll-cell protoplasts and other contaminants by density-gradient centrifugation, and the purity of the preparations carefully and quantitatively assessed by light microscopy. The preparations of guard-cell protoplasts were then compared with mesophyll-cell protoplasts in terms of the activity of photosystem II as assessed by the light-induced evolution of O2 under both steady-state and flashing light and the characteristics of photosystem-II chlorophyll fluorescence. In all experiments, clear photosystem-II activity was found in guard-cell protoplasts, although some subtle distinctions between guard-cell and mesophyll-cell protoplasts were found. The contribution of any contamination by mesophyll-cell chlorophyll to guard-cell-protoplast signals was estimated to be less than 3% in all cases. Photosystem II is apparently present and active in guard cells of Commelina.

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