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The primary sequence of rabbit alpha globin messenger rna


Cell 15(1): 43-54
The primary sequence of rabbit alpha globin messenger rna
The rabbit .alpha.-globin DNA insertion in the chimeric plasmid pHb72 (Liu et al.) was sequenced by the method of Maxam and Gilbert. The mRNA sequence beginning in the 5' untranslated region 9 nucleotides before the initiation codon and extending through the 1st 361 nucleotides of the translated region was determined. The data overlap and are in complete agreement with sequences determined by Baralle for the 5' end of the mRNA and by Proudfoot et al. for the 3' end. The sequence is in agreement with the partial complementary[c]RNA sequencing data reported previously (Paddock et al.) and with the published amino acid sequence. Save for 3 uncertain nucleotides in the 3' sequence determined by Proudfoot et al. this work marks the completion of the primary sequence of the rabbit .alpha.-globin mRNA. The high fidelity with which gene copies can be synthesized in vitro, cloned in a bacterial plasmid and maintained in the host is reaffirmed. The general features of the mRNA nucleotide sequence are discussed with particular attention given to the base composition and codon preferences observed and to comparison of this sequence with other completed mRNA gene sequences. A new computer program was used to search for the most stable base-pairing arrangement of the completed mRNA.


Accession: 006740798



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