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The primary sequence of rabbit alpha globin messenger rna


, : The primary sequence of rabbit alpha globin messenger rna. Cell 15(1): 43-54

The rabbit .alpha.-globin DNA insertion in the chimeric plasmid pHb72 (Liu et al.) was sequenced by the method of Maxam and Gilbert. The mRNA sequence beginning in the 5' untranslated region 9 nucleotides before the initiation codon and extending through the 1st 361 nucleotides of the translated region was determined. The data overlap and are in complete agreement with sequences determined by Baralle for the 5' end of the mRNA and by Proudfoot et al. for the 3' end. The sequence is in agreement with the partial complementary[c]RNA sequencing data reported previously (Paddock et al.) and with the published amino acid sequence. Save for 3 uncertain nucleotides in the 3' sequence determined by Proudfoot et al. this work marks the completion of the primary sequence of the rabbit .alpha.-globin mRNA. The high fidelity with which gene copies can be synthesized in vitro, cloned in a bacterial plasmid and maintained in the host is reaffirmed. The general features of the mRNA nucleotide sequence are discussed with particular attention given to the base composition and codon preferences observed and to comparison of this sequence with other completed mRNA gene sequences. A new computer program was used to search for the most stable base-pairing arrangement of the completed mRNA.

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Related references

Proudfoot, N.J.; Gillam, S.; Smith, M.; Longley, J.I., 1977: Nucleotide sequence of the 3 prime terminal 3rd of rabbit alpha globin messenger rna comparison with human alpha globin messenger rna. Rabbit and human .alpha.-globin complementary DNA was synthesized using the primer (dT1O)dGdCdC hybridized to globin mRNA with reverse transcriptase, and sequenced using the plus and minus gel sequencing procedure. The complete 3' noncoding r...

Proudfoot, N.J.; Gillam, S.; Smith, M.; Longley, J.I., 1977: Nucleotide sequence of the 3' terminal third of rabbit alpha-globin messenger RNA: comparison with human alpha-globin messenger RNA. Rabbit and human alpha-globin complementary DNA was synthesized using the primer (dT10)dGdCdC hybridized to globin mRNA with reverse transcriptase, and sequenced using the plus and minus gel sequencing procedure (Sanger and Coulson, 1975; Brownlee...

Proudfoot, N.J., 1976: Sequence analysis of the 3 prime noncoding regions of rabbit alpha globin and beta globin messenger rna. The sequences of 34 and 75 nucleotides adjacent to the 3' terminal poly(A) sequence of rabbit .alpha.- and .beta.-globin messenger RNA, respectively, were determined. A new method was used to establish these 2 sequences. Short DNA transcripts...

Baralle F.E., 1977: Structure function relationship of 5 prime noncoding sequence of rabbit alpha globin and beta globin messenger rna. Nature (London) 267(5608): 279-281

Tahar S.B.; Scherrer K., 1983: Determination of the primary sequence of the duck alpha d globin messenger rna and comparison of all adult duck and chick globin messenger rna sequences. Molecular Biology Reports 9(1-2): 101-114

Forget B.G.; Marotta C.A.; Cohen Solal M.; Wilson J.T.; Weissman S.M., 1980: Partial nucleotide sequences of human alpha globin messenger rna derived from analysis of phosphorus 32 labeled globin complementary rna and iodine 125 labeled messenger rna correlation with alpha globin complementary dna sequence. The analysis of fingerprints of T1 and pancreatic RNase digests of labeled human globin c[complementary]RNA and mRNA closely supports the nucleotide sequence of human .alpha. globin mRNA that was independently derived by analysis of cloned or de n...

Proudfoot, N.J.; Longley, J.I., 1976: The 3 prime terminal sequences of human alpha and beta globin messenger rna comparison with rabbit globin messenger rna. Sequences of 43 and 70 nucleotides adjacent to the 3' terminal poly(A) of human .alpha. and .beta. globin mRNAs were established. Sequence analysis of complementary DNA from both normal and thalassemic globin mRNA preparations was carried out...

Proudfoot, N.J.; Longley, J.I., 1976: The 3' terminal sequences of human alpha and beta globin messenger RNAs: comparison with rabbit globin messenger RNA. Sequences of 43 and 70 nucleotides adjacent to the 3' terminal poly(A) of human alpha and beta globin mRNAs have been established. Sequence analysis of complementary DNA from both normal and thalassemic globin mRNA preparations was carried ou...

Proudfoot, N.J., 1976: Sequence analysis of the 3' non-coding regions of rabbit alpha- and beta-globin messenger RNAs. Journal of Molecular Biology 107(4): 491-525

Mezl V.A.; Kawasaki E.S.; Hunt J.A., 1979: Analysis of the ratio of alpha globin to beta globin and globin messenger rna content of fractionated rabbit erythroid bone marrow cells. The ratio of .alpha.- to .beta.-globin mRNA was measured by hybridization of a constant amount of highly purified .alpha.- or .beta.-globin (complementary) DNA with increasing amounts of RNA in the range up to 20% cDNA hybridization, where an esse...