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The primary structure of a peroxisomal fatty acyl coenzyme a oxidase from the yeast candida tropicalis pk223






Gene (Amsterdam) 51(2-3): 119-128

The primary structure of a peroxisomal fatty acyl coenzyme a oxidase from the yeast candida tropicalis pk223

We report the isolation and nucleotide (nt) sequence determination of a gene encoding peroxisomal fatty acyl-CoA oxidase (AOx) from the yeast Candida tropicalis pK233. The AOx gene contains no intervening sequences and has a single open reading frame of 2127 nt encoding a protein of 708 amino acids (aa), not including the initiator methionine. The Mr of the protein is 79,155. Codon utilization in the gene is not random, with 87.4% of the aa specified by 25 principal codons. The principal codons used in the expression of AOx in C. tropicalis are similar to those used in highly expressed genes of Saccharomyces cerevisiae. The AOx protein shows a 94.2% homology with POX4 protein of C. tropicalis. One stretch of 36 aa shows no homology between the two proteins.


Accession: 006740834



Related references

Murray W.W.; Rachubinski R.A., 1986: Primary structure of a third peroxisomal fatty acyl coenzyme a oxidase of the yeast candida tropicalis. Journal of Cell Biology 103(5 PART 2): 524A

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