EurekaMag.com logo
+ Translate

The primary structure of chicken liver cytochrome b 5 deduced from the dna sequence of complementary dna clone


, : The primary structure of chicken liver cytochrome b 5 deduced from the dna sequence of complementary dna clone. Archives of Biochemistry & Biophysics 264(1): 343-347

A cDNA clone encoding the chicken liver cytochrome b5 was isolated by probing a library with synthetic oligonucleotides based on a partial amino acid sequence of the protein. Determination of the DNA sequence indicated a 414-nucleotide open reading frame which encodes a 138-amino acid residue polypeptide. The open reading frame contains 6 amino acids at the amino terminus which were not present on any of the cytochorme b5 polypeptides for which the amino acid sequence has been determined directly, suggesting that the protein is proteolytically processed to the mature form. The results of genomic Southern analysis were consistent with the presence of two structurally different genes in the chicken genome, raising the possibility that the soluble and membrane-bound forms of the protein are the products of separate genes.

(PDF 0-2 workdays service)

Accession: 006740877

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Zhang, H.; Somerville, C., 1988: The primary structure of chicken liver cytochrome b5 deduced from the DNA sequence of a cDNA clone. A cDNA clone encoding the chicken liver cytochrome b5 was isolated by probing a library with synthetic oligonucleotides based on a partial amino acid sequence of the protein. Determination of the DNA sequence indicated a 414-nucleotide open readin...

Takai T.; Yokoyama C.; Wada K.; Tanabe T., 1988: Primary structure of chicken liver acetyl coenzyme a carboxylase deduced from complementary dna sequence. The complete amino acid sequence of acetyl-CoA carboxylase from chicken liver has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by Edman degradation of peptide fragments obtaine...

Volokita M.; Somerville C.R., 1987: The primary structure of spinach glycolate oxidase deduced from the dna sequence of complementary dna clone. A cDNA clone encoding the peroxisomal enzyme glycolate oxidase (EC 1.1.3.15) was identified by probing a cDNA library of spinach with synthetic oligonucleotides based on the partial amino acid sequence of the enzyme. Determination of the DNA seque...

Fawcett T.W.; Browse J.A.; Volokita M.; Bartlette S.G., 1990: Spinach carbonic anhydrase primary structure deduced from the sequence of a complementary dna clone. A cDNA clone 1156 base pairs in length was selected by screening a .lambda.gt11 library with antibodies directed against spinach chloroplast carbonic anhydrase (carbonate dehydratase, EC 4.2.1.1). Sequence analysis revealed an open reading frame o...

Kodama R.; Agata K.; Mochii M.; Eguchi G., 1990: Partial amino acid sequence of the major intrinsic protein mip of the chicken lens deduced from the nucleotide sequence of a complementary dna clone. Experimental Eye Research 50(6): 737-742

Noda C.; Ito K.; Nakamura T.; Ichihara A., 1988: Primary structure of rat liver serine dehydratase deduced from the complementary dna sequence. Febs Letters 234(2): 331-335

Oleinikov A.V.; Jokhadze G.G.; Alakhov Y.B., 1989: Primary structure of rat liver elongation factor 2 deduced from the complementary dna sequence. Febs Letters 248(1-2): 131-136

Oka S.; Itoh N.; Kawasaki T.; Yamashina I., 1987: Primary structure of rat liver mannan binding protein deduced from its complementary dna sequence. cDNA clones encoding rat liver mannan-binding protein (MBP), a lectin specific for mannose and N-acetylglucosamine, were isolated from a rat liver cDNA library carried in .lambda.gt 11, by screening with affinity purified polyclonal rabbit anti-ra...

Watahiki M.; Tanaka M.; Masuda N.; Sugisaki K.; Yamamoto M.; Yamakawa M.; Nagai J.; Nakashima K., 1989: Primary structure of chicken pituitary prolactin deduced from the complementary dna sequence conserved and specific amino acid residues in the domains of the prolactins. The preform of chicken prolactin (PRL) deduced from the cDNA sequence contains a signal peptide of 30 amino acid residues followed by a mature PRL of 199 residues. Chicken PRL shows 77, 68, 67, 58, and 31% identity of amino acid sequence with whal...

Takai, T.; Yokoyama, C.; Wada, K.; Tanabe, T., 1988: Primary structure of chicken liver acetyl-CoA carboxylase deduced from cDNA sequence. The 7368-nucleotide cDNA sequence of acetyl-CoA carboxylase is presented.