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The primary structure of chicken liver cytochrome b 5 deduced from the dna sequence of complementary dna clone


Archives of Biochemistry & Biophysics 264(1): 343-347
The primary structure of chicken liver cytochrome b 5 deduced from the dna sequence of complementary dna clone
A cDNA clone encoding the chicken liver cytochrome b5 was isolated by probing a library with synthetic oligonucleotides based on a partial amino acid sequence of the protein. Determination of the DNA sequence indicated a 414-nucleotide open reading frame which encodes a 138-amino acid residue polypeptide. The open reading frame contains 6 amino acids at the amino terminus which were not present on any of the cytochorme b5 polypeptides for which the amino acid sequence has been determined directly, suggesting that the protein is proteolytically processed to the mature form. The results of genomic Southern analysis were consistent with the presence of two structurally different genes in the chicken genome, raising the possibility that the soluble and membrane-bound forms of the protein are the products of separate genes.


Accession: 006740877



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