+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The primary structure of coho salmon growth hormone and its complementary dna

, : The primary structure of coho salmon growth hormone and its complementary dna. General & Comparative Endocrinology 68(3): 387-399

Total RNA was extracted from coho salmon growth hormone (sGH) cell regions and used to synthesize double-stranded cDNA, which was inserted into a plasmid vector used to transform Escherichia coli HB101. The total RNA as also separated according to size by electrophoresis on agarose gels and the fraction that directed the cell-free synthesis of protein in the size range of GHs of other species was isolated and used to screen the transformed colonies of E. coli. A clone containing the putative sGH cDNA was identified and its nucleotide sequence was determined. To verify that the cDNA was that of sGH, the GH cell region of coho pituitary glands was incubated in organ culture. The secreted GH was purified by HPLC and the sequence of its 42 amino-terminal amino acids was determined. Comparison of this sequence with the amino acid sequence derived from the cDNA showed that it encoded sGH. Medium containing the presumptive sGH as the only prominent protein was active in a GH radioreceptor assay that involved labeled bovine GH and pregnant mouse liver membranes. The sGH was approximately 10% as active as the bGH standard. RNA blotting analysis showed that sGH was the major species of RNA produced by the GH cell region of the salmon pituitary. The mRNA of sGH differed from those of human, rat, and bovine GH in that its 3'-untranslated region was unusually large (about 500 nucleotides) but the coding region showed significant homology with mammalian GHs and resembled them in having a strong (78%) preference for G and C in the third positions of the codons. The amino acid sequence of sGH showed 32-34% and 19-22% identical homology with mammalian GHs and prolactins, respectively. Several conserved regions between sGH and mammalian GH and PRL molecules were also revealed that could indicate conservation of structurally and/or functionally important domains. Hydropathy analysis disclosed that although sGH and the GH of a representative mammal (pig) had similar profiles in some regions, the sGH was overall more hydrophobic than the pig (p) GH. Similarities and differences were also noted in the predicted secondary structure sGH and pGH.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006740880

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Beck, T.W.; Housh, T.J.; Malek, M.H.; Mielke, M.; Hendrix, R., 2008: The acute effects of a caffeine-containing supplement on bench press strength and time to running exhaustion. The purpose of the present study was to examine the acute effects of a caffeine-containing supplement (SUPP) on one-repetition maximum (1-RM) bench press strength and time to running exhaustion (TRE) at a velocity that corresponded to 85% of the p...

Yamashina, M.; Wilson, T.K., 1985: A mammographic finding in focal polyarteritis nodosa. British Journal of Radiology 58(685): 91-92

Okoshi T.; Endo M.; Hashimoto A.; Koyanagi H., 1987: Usefulness and safety of the intraluminal ringed graft in aortic surgery. Journal of Cardiovascular Surgery 28(5 SUPPL): 105

Knox, K.; Moore, J.S., 2001: Predictive validity of the Strain Index in turkey processing. The Strain Index is a job analysis method for determining if workers are exposed to increased risk of developing distal upper extremity disorders. Its predictive and external validity was initially demonstrated in a pork processing plant. The purp...

Khadem-Rezaiyan, M.; Moallem, S.Reza.; Vakili, V., 2016: High-risk behaviors while driving: A population-based study from Iran. Traffic injuries are becoming one of the most important challenges of public health systems. Because these injuries are mostly preventable, the aim of this study is to evaluate the four main high-risk behaviors while driving. This cross-sectional...

Talbot, R.; L.G.ange, L., 2003: The Effects of Salty and Nonsalty Food on Peak Breath Alcohol Concentration and Divided Attention Task Performance in Women. There is recent evidence that gender-based patterns of alcohol consumption are becoming less dissimilar and that, per capita, women are consuming more alcohol than ever before. Therefore, a better understanding of the acute effects of moderate alc...

Tiffee, J.C.; Aufdemorte, T.B., 1997: Markers for macrophage and osteoclast lineages in giant cell lesions of the oral cavity. Giant cell lesions of the oral cavity are a well recognized entity. However, the histogenesis of these lesions is still the subject of controversy, with support for both histiocyte/macrophage and osteoclast origins being found in the literature. T...

Nungesser, W.C.; Hiatt, E.P., 1954: The action of veratrum on renal circulation of the dog. The effects of single intraven. injns. of Veratrone upon the blood pressure and renal hemodynamics of anesthetized dogs having one kideny acutely denervated were studied. Data from 6 8-hr. expts. are presented. Changes in renal plasma flow and gol...

Pritchard, D..; Haggett, A.., 1998: Melanistic ovambo sparrowhawk Accipiter ovampensis at Shakawe Lodge. Babbler (Gaborone). June; 33: 30

Haag, C..; Depenbusch, M.., 1995: Carabiden auf forstlichen Rekultivierungsflachen des Rheinischen Braunkohlentagebaus: Besiedlung, Reproduktion und Sukzession. Mitteilungen der Deutschen Gesellschaft fuer Allgemeine und Angewandte Entomologie. September; 94-6: 727-731