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The primary structure of d amino acid oxidase ec 1.4.3.3 from pig kidney 1. isolation and sequence of the tryptic peptides


, : The primary structure of d amino acid oxidase ec 1.4.3.3 from pig kidney 1. isolation and sequence of the tryptic peptides. Journal of Biological Chemistry 257(15): 8817-8823

D-Amino acid oxidase from pig kidney cortex was digested with trypsin. Thirty-two tryptic peptides were isolated by ion exchange chromatography, high voltage paper electrophoresis, descending paper chromatography, and reverse-phase high performance liquid chromatography. The last method permitted the isolation of 29 tryptic peptides, many in a single step, in yields usually > 75%. The purified peptides were characterized by amino acid analysis and their sequences determined by the manual 5-dimethylaminonaphthalene-1-sulfonyl-Edman degradation procedure or by the automated Edman-Begg degradation method. These peptides accounted for all 12 lysine and 21 arginine residues observed by amino acid analysis of the intact protein and for 347 amino acid residues of the 345 predicted by the analysis.

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Swenson, R.P.; Williams, C.H.; Massey, V.; Ronchi, S.; Minchiotti, L.; Galliano, M.; Curti, B., 1982: The primary structure of D-amino acid oxidase from pig kidney. I. Isolation and sequence of the tryptic peptides. D-Amino acid oxidase from pig kidney cortex was digested with trypsin. Thirty-two tryptic peptides were isolated by ion exchange chromatography, high voltage paper electrophoresis, descending paper chromatography, and reverse-phase high performanc...

Ronchi, S.; Minchiotti, L.; Galliano, M.; Curti, B.; Swenson, R.P.; Williams, C.H.; Massey, V., 1982: The primary structure of D-amino acid oxidase from pig kidney. II. Isolation and sequence of overlap peptides and the complete sequence. Journal of Biological Chemistry 257(15): 8824-8834

Ronchi, S.; Minchiotti, L.; Galliano, M.; Curti, B.; Swenson, R.P.; Williams, C.H.Jr ; Massey, V., 1982: The primary structure of d amino acid oxidase ec 1.4.3.3 from pig kidney 2. isolation and sequence of overlap peptides and the complete sequence. The complete primary structure of D-amino acid oxidase from pig kidney was determined by sequence analysis of 32 peptides derived from tryptic digests of the protein which were aligned by peptides derived from chymotryptic and Staphylococcus aureu...

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