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The primary structure of human chromogranin A and pancreastatin

, : The primary structure of human chromogranin A and pancreastatin. Journal of Biological Chemistry 262(35): 17026-17030

A full-length clone encoding human chromogranin A has been isolated from a .lambda.gt10 cDNA library of a human pheochromocytoma. The nucleotide sequence reveals that human chromogranin A is a 439-residue protein preceded by an 18-residue signal-peptide. Comparison of the protein sequence of human chromogranin A with that of bovine chromogranin A shows high conservation of the NH2-terminal and COOH-terminal domains as well as the potential dibasic cleavage sites, whereas the middle portion shows remarkable sequence variation (36%). This part of human chromogranin A contains a sequence homologous to porcine pancreastatin at residues 250-301. The sequence variation in this part of human chromogranin A compared to porcine pancreastatin is 32% and thus of the same magnitude as that between human and bovine chromogranin A. Therefore, the difference between porcine pancreastatin and the corresponding portions of bovine or human chromogranin A can be explained by species variation, suggesting that pancreastatin is derived from chromogranin A itself rather than a protein that is only similar to chromogranin A. Moreover, the pancreastatin sequenced contained in human chromogranin A is flanked by sites for proteolytic processing. Together, thse observations suggest that human chromogranin A may be the precursor for a human pancreastatin molecule and possibly for other, as yet unidentified, biologically active peptides.

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Accession: 006740915

PMID: 2445752

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George, C. F., 1914: A new Trombidium Ottonia ignota n sp. Naturalist London, 306

Daborn, GR.; Gregory, RS., 1983: Occurrence, distribution, and feeding habits of juvenile lumpfish, Cyclopterus lumpus L. in the Bay of Fundy. Juvenile lumpfish (Cyclopterus lumpus) (<55 mm length) feed on a wide range of near-surface plankton, switching with growth from harpacticoid copepods (particularly Harpacticus chelifer) to the amphipods Calliopius laeviusculus and Parathemisto...

Gothel, Helmut., 1994: Farbatlas Meeresfauna: Rotes Meer, Indischer Ozean (Malediven). Band 2. Fische. Unknown

Siegel F. R.; Hearn P. P. Jr.; Pierce J. W., 1986: Geochemistry of Chilean Archipelago bottom sediments, 55 degrees -42 degrees latitude. Antarctic Journal of the United States 21(5): 139-140

Querfurth H.W.; Mammen A.; Mellon A.; Selkoe D., 1992: Transcriptional control of the beta amyloid precursor gene in fad lymphoblasts and fibroblasts. Neurology 42(4 SUPPL 3): 303-304

Roch, J-F., 1979: Carabidae au Camp Rolland-Germain a Frelighsburg, Quebec. Bulletin d'Inventaire des Insectes du Quebec, 13: 44

Ford, Derek, 2008: Morphology and genesis of the main ore body at Nanisivik zinc/lead mine, Baffin Island, Canada; an outstanding example of paragenetic dissolution of carbonate bedrocks with pene-contemporaneous; precipitation of sulfides and gangue minerals in a hypogene setting. Karst Waters Institute Special Publication 14: 76-80

Xiao-Ping Ruan,Ji-Zheng Mao,Zhan-Tao Cui, 1993: Laboratory investigation of borehole breakouts and multi-step failure model. Based on our experiment of borehole breakouts with a group of sandstone samples described in this paper, a multi-step failure model of borehole breakouts are proposed to quantitatively explain the relationship between the section shape of borehole...

Ting, Irwin P.; Loomis, Walter E., 1965: The effect of wind on transpiration and evaporation through multiperforate septa. Diffusion of water vapor through single-pore membranes with pores 100 to 800 [mu] in diameter and multipore membranes with pores 2.5 to 20 [mu] in diameter was studied as a function of wind velocity. The results of these studies are compared with...

Fujii, Yuichi; Johnson, Michael E.; Gores, Gregory J., 1994: Mitochondrial dysfunction during anoxia/reoxygenation injury of liver sinusoidal endothelial cells. Sinusoidal endothelial cell injury plays a pivotal role in anoxia/reoxygenation liver damage. However, the mechanisms culminating in anoxia/reoxygenation endothelial cell injury remain unclear. Our aims were to determine whether anoxia/reoxygenati...