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The primary structure of human erythrocyte copper zinc super oxide dis mutase ec 1.15.1.1 cleavage with trypsin and chymotrypsin


, : The primary structure of human erythrocyte copper zinc super oxide dis mutase ec 1.15.1.1 cleavage with trypsin and chymotrypsin. Italian Journal of Biochemistry (English Edition) 31(1): 28-38

Results obtained after digestion of copper/zinc superoxide dismutase from human erythrocytes with trypsin and chymotrypsin are described. Tryptic digestion produced a relatively simple mixture of soluble peptides, which were purified and analyzed mainly by conventional techniques. The more complex mixture of chymotryptic peptides was treated similarly. Combined structural information from the 2 digests provided evidence for 82.4% of the total sequence.

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