EurekaMag.com logo
+ Translate

The primary structure of leucine transfer rna from cow mammary gland oligo nucleotides of t 1 rnase hydrolysate the reconstruction of the total nucleotide sequence


, : The primary structure of leucine transfer rna from cow mammary gland oligo nucleotides of t 1 rnase hydrolysate the reconstruction of the total nucleotide sequence. Bioorganicheskaya Khimiya 10(1): 58-67

The oligonucleotides obtained by digestion of leucine transfer RNA from cow mammary gland with T1 RNase were separated by micro-column chromatography on DEAE-cellulose in 7 M urea at pH 7.5 and 3.7, and in addition on Dowex 1 .times. 2. The digest consisted of 18 individual components, the larger being a tridecanucleotide. Micro-column chromatography of nucleotides on anion-exchanger AG 1 .times. 8 and nucleosides on Aminex A-6 was used to determine the base composition of the oligonucleotides. The oligonucleotide structure was established using terminal analysis, hydrolysis by pyrimidyl and U2-RNases and incomplete hydrolysis by snake venom phosphodiesterases. The total primary structure of leucine transfer RNA was derived from overlapping fragments isolated after its complete hydrolysis with pancreatic and T1 RNase and using data obtained on S1-nuclease digestion of tRNA. The methods of rapid gel-sequencing were also employed for checking the nucleotide sequence of leucine transfer RNA from cow mammary gland.

(PDF 0-2 workdays service)

Accession: 006740936

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Vlasov V.V., 1984: Primary structure of bovine mammary gland leucine transfer rna the oligonucleotides of an rnase t 1 hydrolysate reconstruction of the complete nucleotide sequence. Soviet Journal Of Bioorganic Chemistry: 31-39

Tukalo, M.A.; Vasil'eva, I.G.; Matsuka, G.Kh.; Vlasov, V.B., 1984: Primary structure of tRNA2Leu of bovine mammary gland. Oligonucleotides of T1-RNAse hydrolysate. Reconstruction of a total nucleotide sequence. The oligonucleotides obtained by digestion of tRNA2Leu from cow mammary gland with T1 RNase were separated by micro-column chromatography on DEAE-cellulose in 7 M urea at pH 7,5 and 3,7, and in addition on Dowex 1 x 2. The digest consisted of 18 i...

Tukalo M.A.; Vasil'eva I.G.; Matsuka G.Kh; Vlasov V.V., 1984: The primary structure of leucine transfer rna from cow mammary gland structure determination for oligo nucleotides of the pyrimidyl ribo nuclease hydrolysate by micro spectrophotometric methods. tRNA2Leu from cow mammary gland was degraded with RNase, and the fragments obtained were separated by DEAE-cellulose micro-column chromatography in 7 M urea at pH 7.5. Rechromatography was performed on a DEAE-cellulose microcolumn at pH 3.7 and al...

Tukalo M.A.; Vasil'eva I.G.; Matsuka G.Kh; Vlasvo V.V., 1984: Primary structure of bovine mammary gland leucine transfer rna determination of the structure of the oligonucleotides of a pyrimidyl rnase hydrolysate of leucine transfer rna by microspectrophotometric methods. Soviet Journal of Bioorganic Chemistry 10(1): 24-30

Tukalo, M.; Vasil'-Eva, I.; Matsuka, G.; Vlasov, V., 1984: Primary structure of bovine mammary gland tRNA2Leu. The oligonucleotides of a RNase T1 hydrolysate. Reconstruction of the complete nucelotide sequence. Soviet journal of bioorganic chemistry 10(1): 31-39

Mirzabekov A.Baev A.A., 1968: Primary structure of valine transfer rna 1 1 oligo nucleotides of enz pyrimidyl rnase hydrolysate saccharomyces cerevisiae. Molekulyarnaya Biologiya (moscow): 394-407

Kryukov, V.M.; Gorbulev, V.G.; Akselrod, V.D., 1976: Primary structure of valine transfer rna 2a from bakers yeast oligo nucleotides of guanyl rnase hydrolysate. The oligonucleotides obtained by the digestion of .**GRAPHIC**. from baker's yeast [Saccharomyces cerevisiae] with guanyl-RNase were separated by column chromatography on DEAE-cellulose in 7 M urea at pH 7.4 and 3.5. The digest consists of 18...

Tukalo, M.A.; Vasil' eva, I.G.; Matsuka, G.K.; Vlasov, V.V., 1984: Primary structure of bovine mammary gland tRNA2Leu. The oligonucleotides of a RNAase T1 hydrolysate. Reconstruction of the complete nucleotide sequence. The products of the hydrolysis of bovine mammary gland leucine tRNA2 by RNase T1 [see preceding abstr.] were separated on microcolumns containing DEAE-cellulose in 7M urea at pH 7.5 with subsequent rechromatography at pH 3.7. The oligonucleotides...

Isaenko, S.N.; Aksel'rod, V.D., 1976: Primary structure of transfer valine transfer rna 2a from bakers yeast oligo nucleotides of the complete pyrimidyl rnase hydrolysate of valine transfer rna 2a. Valine 2a transfer RNA .**GRAPHIC**. from baker's yeast [Saccharomyces cerevisiae] was degraded with pancreatic RNase, and the fragments obtained were separated by DEAE-cellulose chromatography in 7 M urea at pH 7.4. Rechromatography of some...

Marcker K.A., 1969: The nucleotide sequence of n formyl methionyl transfer rna partial digestion with pancreatic enz rnase and t 1 enz rnase and derivation of the total primary structure escherichia coli. European Journal Of Biochemistry: 256-262