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The primary structure of leucine transfer rna from cow mammary gland structure determination for oligo nucleotides of the pyrimidyl ribo nuclease hydrolysate by micro spectrophotometric methods


, : The primary structure of leucine transfer rna from cow mammary gland structure determination for oligo nucleotides of the pyrimidyl ribo nuclease hydrolysate by micro spectrophotometric methods. Bioorganicheskaya Khimiya 10(1): 50-57

tRNA2Leu from cow mammary gland was degraded with RNase, and the fragments obtained were separated by DEAE-cellulose micro-column chromatography in 7 M urea at pH 7.5. Rechromatography was performed on a DEAE-cellulose microcolumn at pH 3.7 and also on Dowex 1 .times. 2 in a formiate system. Nucleotide analysis was carried out with the aid of T2-RNase hydrolysis followed by chromatography on anion-exchanger AG 1 .times. 8. Nucleosides were separated on Aminex A-6 at pH 9.8. Fifteen minor components were present: T, 2 .psi., 2 Um, 2D, m5C, ac4C, m1G, 2m2G, m22G, m1A and N, the N is not identified so far. The structure of oligonucleotides was established by terminal analysis, hydrolysis with T1-RNase and also using incomplete hydrolysis by the snake venom phosphodiesterase.

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