EurekaMag.com logo
+ Translate

The primary structure of leucine transfer rna from cow mammary gland structure determination for oligo nucleotides of the pyrimidyl ribo nuclease hydrolysate by micro spectrophotometric methods


, : The primary structure of leucine transfer rna from cow mammary gland structure determination for oligo nucleotides of the pyrimidyl ribo nuclease hydrolysate by micro spectrophotometric methods. Bioorganicheskaya Khimiya 10(1): 50-57

tRNA2Leu from cow mammary gland was degraded with RNase, and the fragments obtained were separated by DEAE-cellulose micro-column chromatography in 7 M urea at pH 7.5. Rechromatography was performed on a DEAE-cellulose microcolumn at pH 3.7 and also on Dowex 1 .times. 2 in a formiate system. Nucleotide analysis was carried out with the aid of T2-RNase hydrolysis followed by chromatography on anion-exchanger AG 1 .times. 8. Nucleosides were separated on Aminex A-6 at pH 9.8. Fifteen minor components were present: T, 2 .psi., 2 Um, 2D, m5C, ac4C, m1G, 2m2G, m22G, m1A and N, the N is not identified so far. The structure of oligonucleotides was established by terminal analysis, hydrolysis with T1-RNase and also using incomplete hydrolysis by the snake venom phosphodiesterase.

(PDF 0-2 workdays service)

Accession: 006740937

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Tukalo M.A.; Vasil'eva I.G.; Matsuka G.Kh; Vlasvo V.V., 1984: Primary structure of bovine mammary gland leucine transfer rna determination of the structure of the oligonucleotides of a pyrimidyl rnase hydrolysate of leucine transfer rna by microspectrophotometric methods. Soviet Journal of Bioorganic Chemistry 10(1): 24-30

Tukalo M.A.; Vasil'eva I.G.; Matsuka G.Kh; Vlasov V.V., 1984: The primary structure of leucine transfer rna from cow mammary gland oligo nucleotides of t 1 rnase hydrolysate the reconstruction of the total nucleotide sequence. The oligonucleotides obtained by digestion of leucine transfer RNA from cow mammary gland with T1 RNase were separated by micro-column chromatography on DEAE-cellulose in 7 M urea at pH 7.5 and 3.7, and in addition on Dowex 1 .times. 2. The digest...

Mirzabekov A.Baev A.A., 1968: Primary structure of valine transfer rna 1 1 oligo nucleotides of enz pyrimidyl rnase hydrolysate saccharomyces cerevisiae. Molekulyarnaya Biologiya (moscow): 394-407

Tukalo, M.A.; Vasil' eva, I.G.; Matsuka, G.K.; Vlasov, V.V., 1984: Primary structure of bovine mammary gland tRNA2Leu. Determination of the structure of the oligonucleotides of a pyrimidyl-RNase hydrolysate of tRNA2Leu by microspectrophotometric methods. Oligonucleotides of the pyrimidyl-RNase hydrolysate of leucine tRNA2 of the bovine mammary gland were separated by microcolumn chromatography on DEAE-cellulose at pH 7.5 and 3.7 and also on Dowex 1 x 2 in the formate system. The structures of thes...

Isaenko, S.N.; Aksel'rod, V.D., 1976: Primary structure of transfer valine transfer rna 2a from bakers yeast oligo nucleotides of the complete pyrimidyl rnase hydrolysate of valine transfer rna 2a. Valine 2a transfer RNA .**GRAPHIC**. from baker's yeast [Saccharomyces cerevisiae] was degraded with pancreatic RNase, and the fragments obtained were separated by DEAE-cellulose chromatography in 7 M urea at pH 7.4. Rechromatography of some...

Knorre D.G., 1973: Micro analysis of the oligo nucleotides of pyrimidyl rnase hydrolysate of 5 half of valine transfer rna modified with alkylating derivative of adenylic acid. Molekulyarnaya Biologiya (moscow): 12-19

Vlasov V.V., 1984: Primary structure of bovine mammary gland leucine transfer rna the oligonucleotides of an rnase t 1 hydrolysate reconstruction of the complete nucleotide sequence. Soviet Journal Of Bioorganic Chemistry: 31-39

Tukalo M.A.; Vlasov V.V.; Vasil'chenko I.G.; Matsuka G.Kh; Knorre D.G., 1980: Primary structure of leucine transfer rna 2 from the cow mammary gland according to the data of ultra micro spectrophotometry. Doklady Biochemistry 253(1-6): 222-225

Tukalo, M.A.; Vasil'eva, I.G.; Matsuka, G.Kh.; Vlasov, V.V., 1984: Primary structure of tRNA2Leu from the bovine udder. Determination of the oligonucleotide structure of the pyrimidyl-RNAse hydrolysate of tRNA2Leu by microspectrophotometric methods. tRNA2Leu from cow mammary gland has been degraded with pancreatic ribonuclease, and the fragments obtained were separated by DEAE-cellulose micro-column chromatography in 7 M urea at pH 7,5. Rechromatography was performed on a DEAE-cellulose micro...

Kryukov, V.M.; Gorbulev, V.G.; Akselrod, V.D., 1976: Primary structure of valine transfer rna 2a from bakers yeast oligo nucleotides of guanyl rnase hydrolysate. The oligonucleotides obtained by the digestion of .**GRAPHIC**. from baker's yeast [Saccharomyces cerevisiae] with guanyl-RNase were separated by column chromatography on DEAE-cellulose in 7 M urea at pH 7.4 and 3.5. The digest consists of 18...