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The primary structure of messenger rna from escherichia coli constraints on nucleotides on the 3' side of codons

, : The primary structure of messenger rna from escherichia coli constraints on nucleotides on the 3' side of codons. Molekulyarnaya Biologiya (Moscow) 19(3): 791-799

The occurrence of nucleotides of the 3' side of codons has been determined in highly and weakly expressed genes from Escherichia coli. It was found that the usage of some amino acid condons in highly expressed genes was site specific, depending on the base 3' to the codon. The role of the 3' nucleotide as a modulator of codon translation effectiveness is discussed. The rules of synonymous codon usage in relation to the 3' flanking nucleotide have been established for highly expressed genes. For example, if a triplet next to the lysine codon starts with guanosine, lysine is preferably encoded by AAA and not by AAG (P < 10-8), while of cytidine is 3' to the lysine codon, AAG is preferred over AAA (P < 0.001). These rules are observed in highly and absent in weakly expressed mRNAs and can be used in the chemical synthesis of genes designed for expression in E. coli.

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