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The primary structure of noninitiator methionine transfer rna from bakers yeast part 2 partial digestion with rnase t 1 and derivation of the complete sequence


, : The primary structure of noninitiator methionine transfer rna from bakers yeast part 2 partial digestion with rnase t 1 and derivation of the complete sequence. Journal of Biochemistry (Tokyo) 80(5): 951-959

Large oligonucleotides obtained from partial RNase T1 digestion of .**GRAPHIC**. from bakers' yeast (Saccharomyces cerevisiae, strain Y 185) were isolated by chromatographic procedures and sequenced. The complete sequence of the .**GRAPHIC**. was established from the results of this partial digestion, together with those of the complete RNase T1 and A [EC 3.1.4.8 and 22] digestions of .**GRAPHIC**. reported in the preceding paper. The structure of this .**GRAPHIC**. was arranged into a clover leaf comparable with those of other tRNAMet species including bakers' yeast initiator tRNA.

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Koiwai, O.; Miyazaki, M., 1976: The primary structure of noninitiator methionine transfer rna from bakers yeast part 1 purification and complete digestion with rnase t 1 and pancreatic rnase a ec 3.1.4.8. The methionine acceptor activity on a crude tRNA from bakers' yeast [Saccharomyces cerevisiae] was resolved into 2 peaks (I and II) by column chromatography on DEAE-Sephadex A-25 with a 1 M phosphate system. Methionine tRNA from peak II was n...

Koiwai, O.; Miyazaki, M., 1976: The primary structure of non-initiator methionine transfer ribonucleic acid from Bakers' yeast. II. Partial digestion with ribonuclease T1 and derivation of the complete sequence. Large oligonucleotides obtained from partial RNase T1 digestion of tRNAMetm from bakers' yeast (S. cerevisiae, strain Y 185) were isolated by chromatographic procedures and sequenced. The complete sequence of the tRNAMetm was established from...

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