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The primary structure of phosphoenol pyruvate carboxylase ec 4.1.1.31 of escherichia coli nucleotide sequence of the ppc gene and deduced amino acid sequence






Journal of Biochemistry (Tokyo) 95(4): 909-916

The primary structure of phosphoenol pyruvate carboxylase ec 4.1.1.31 of escherichia coli nucleotide sequence of the ppc gene and deduced amino acid sequence

The nucleotide sequence of the ppc gene, the structural gene for phosphoenolypyruvate carboxylase [EC 4.1.1.31], of E. coli K-12 was determined. The gene codes for a polypeptide comprising 883 amino acid residues with a calculated MW of 99,061. The amino acid sequence deduced from the nucleotide sequence was entirely consistent with the protein chemical data obtained with the purified enzyme, including the NH2- and COOH-terminal sequences and amino acid composition. The coding region is preceded by 2 putative ribosome binding sites and is followed closely by a good representative of rho-independent terminator. The codon usage in the ppc gene suggests a moderate expression of the gene. The secondary structure of the enzyme was predicted from the deduced amino acid sequence.


Accession: 006740960



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