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The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
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The proliferative effects of human granulocyte macrophage colony stimulating factor alpha and granulocyte macrophage colony stimulating factor beta and murine granulocyte colony stimulating factor in microwell cultures of fractionated human marrow cells


The proliferative effects of human granulocyte macrophage colony stimulating factor alpha and granulocyte macrophage colony stimulating factor beta and murine granulocyte colony stimulating factor in microwell cultures of fractionated human marrow cells



Leukemia Research 9(5): 521-528



ISSN/ISBN: 0145-2126

Blast cell-enriched and promyelocyte-myelocyte-enriched fractions of human marrow were prepared by fluorescence-activated cell sorting using an antineutrophil monoclonal antiserum. Cells from both fractions proliferated in microwell cultures when stimulated by placental or bladder cancer cell conditioned medium containing colony stimulating factor. Semipurified preparations of both GM-CSF [granulocyte-macrophage colony stimulating factor] .alpha. and .beta. from bladder cancer cell conditioned medium were effective proliferative stimuli for both cell populations. Pure murine G-CSF, GM-CSF, M-CSF and Multi-CSF failed to stimulate detectable proliferation in blast cell fractions containing granulocyte-monocyte progenitors. However, G-CSF was an effective proliferative stimulus for human promyelocytes and myelocytes leading to the formation of differentiating granulocytic progeny. G-CSF also stimulated the proliferation of human promyelocytic leukemic cells. Promyelocyte-myelocyte-enriched fractions of human marrow appear to be useful target cells for monitoring the proliferative effects of human-active CSF in microwell cultures.

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Accession: 006743031

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