The rates of photo phosphorylation of the blue green alga anacystis nidulans at transition from dark to light part 2 dependence of atp formation on ph and phosphate concentration of the medium temperature and light intensity
Biochemie und Physiologie der Pflanzen (BPP) 170(4-5): 345-353
In the blue-green alga A. nidulans ATP formation at transition from dark to light was measured dependent upon pH (5.0-9.5), temperature (0-55.degree. C), light intensity (100-20,000 lx) and phosphate concentration (up to 100 mM). ATP formation shows clearcut dependencies with optimal values at pH 8.0, 45.degree. C, 10,000 lx and phosphate concentration lower than 1 .mu.M. In the presence of 10 .mu.M DCMU [3(3,4-dichlorophenyl)-1,1-dimethylurea] ATP formation becomes saturated at 300 lx. With respect to light intensity and to temperature the curves for ATP formation and [14C]O2 fixation are of comparable shape, while there is an opposite correlation with respect to phosphate concentration.