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The role of ph in the chemi luminescent response of the myelo peroxidase halide hydrogen per oxide anti microbial system



The role of ph in the chemi luminescent response of the myelo peroxidase halide hydrogen per oxide anti microbial system



Biochemical and Biophysical Research Communications 63(3): 684-691



Myeloperoxidase extracted from human polymorphonuclear (PMN) leukocytes yielded a chemiluminescence in the presence of halide cofactor and HOOH. This enzyme has an established pH optimum for microbicidal activity. The effects of varying H+ concentration on the myeloperoxidase system was investigated using the criterion of chemiluminescence, and a pH optimum was established with either Cl- or Br- as cofactor. This optimum was in agreement with that established for antimicrobial action. The electronegative character of the various halides and the effect of various H+ concentrations were considered with respect to halide oxidation, and the role of the oxidized halide-HOOH reaction in the generation of singlet multiplicity molecular oxygen was discussed.

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