+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The synthesis and deposition of the prolamin storage proteins secalins of rye secale cereale cultivar gazelle



The synthesis and deposition of the prolamin storage proteins secalins of rye secale cereale cultivar gazelle



Planta (Heidelberg) 159(5): 439-445



The synthesis and deposition of the endosperm storage proteins of rye, usually termed secalins, was studied. The rate of accumulation of secalin in developing rye grain was at a maximum between 3-5 wk after anthesis. Some changes in the proportions of the 4 major groups of secalin polypeptides were observed during maturation, notably an increase in .gamma.-secalins of MW 75k and a decrease in .omega.-secalins. In-vitro translation of mRNA fractions prepared from 4 wk old endosperms showed that secalin polypeptides were synthesized on membrane-bound polysomes. The secalin products were identified by their mobilities on SDS-PAGE [sodium-dodecyl sulfate polyacrylamide gel electrophoresis] and their relative incorporation of radioactive lysine, glycine, proline, leucine and methionine. Protein bodies prepared by sucrose density ultracentrifugation contained reduced amounts of .gamma.-secalins of MW 40k and .omega.-secalins compared with the total secalin fraction, but these components were present in the expected amounts when 1.0 M NaCl was added to the buffers. Treatment of the protein bodies with proteinase-k resulted in the digestion of their contents regardless of the presence of NaCl, indicating that the surrounding membrane was incomplete. The NaCl reduced the loss of secalins from the protein bodies by decreasing secalin solubility rather than by affecting the integrity of the protein body membrane. The results reported for the synthesis and deposition of secalins are consistent with the results of previous studies on the prolamins of wheat and barley.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006777227

Download citation: RISBibTeXText


Related references

The purification and characterization of 2 groups of storage proteins secalins from rye secale cereale cultivar reidol. Journal of Experimental Botany 33(133): 261-268, 1982

The synthesis and deposition of the prolamin storage proteins (secalins) of rye. Planta 159(5): 439-445, 1983

The Purification and Characterization of Two Groups of Storage Proteins (Secalins) from Rye ( Secale cereale L.). Journal of Experimental Botany 33(2): 261-268, 1982

The structural and evolutionary relationships of the prolamin storage proteins of barley hordeum vulgare rye secale cereale and wheat triticum aestivum. Philosophical Transactions of the Royal Society of London B Biological Sciences 304(1120): 297-308, 1984

Extraction separation and polymorphism of the prolamin storage proteins secalins of rye. Cereal Chemistry 60(1): 1-6, 1983

The chromosomal locations and linkage relationships of the structural genes for the prolamin storage proteins (secalins) of rye. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 69(1): 63-69, 1984

Chromosomal locations of the structural genes for secalins in wild perennial rye secale montanum and cultivated rye secale cereale determined by two dimensional electrophoresis. Canadian Journal of Genetics and Cytology 28(1): 76-83, 1986

Chromosomal location of the structural genes for the Mr 75,000 c-secalins in Secale montanum Guss: evidence for a translocation involving chromosomes 2R and 6R in cultivated rye (Secale cereale L.). Heredity 54: 1-3, 1985

Prolamin synthesis in the developing endosperm of wheat triticum aestivum and rye secale cereale. Canadian Journal of Plant Science 284, 1987

Chromosomal location of the structural genes for the 75000 molecular weight gamma secalins in secale montanum evidence for a translocation involving chromosomes 2r and 6r in cultivated rye secale cereale. Heredity 54(3): 381-383, 1985

Comparison of rye populations secale cereale according to their secalins polymorphism. Cereal Research Communications 12(3-4): 215-222, 1984

Lectin synthesis in developing and germinating wheat triticum vulgare cultivar celesta and rye secale cereale cultivar celestijner embryos. Planta (Heidelberg) 156(1): 41-44, 1982

Changes in the electrophoretic patterns of the soluble proteins of winter wheat triticum aestivum cultivar cappelle desprez and rye secale cereale cultivar puma following cold acclimation and desiccation stress. Plant Physiology 71(2): 400-403, 1983

Relationship between chromosomal aberrations and long term seed storage of rye secale cereale l. cultivar dankowskie zlote. Genetica Polonica 31(2): 89-98, 1990

Polymorphism of the storage proteins in Portuguese rye (Secale cereale L.) populations. Hereditas 149(2): 72-84, 2012