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The use of monoclonal antibodies in elisa for detection of antibodies to bovine viral diarrhea virus



The use of monoclonal antibodies in elisa for detection of antibodies to bovine viral diarrhea virus



Journal of Veterinary Medicine Series B 34(5): 356-363



Two ELISA techniques were developed for the detection of antibodies to bovine viral diarrhoea virus (BVDV), an indirect and a competitive ELISA. The coating antigen was prepared from virus harvests which were 10 to 100 times larger than usual. This was achieved by culturing the cells in a growth medium with foetal calf serum devoid of IgG. The harvested BVDV was solubilized with detergent to increase the accessibility of antigens. By these modifications, the amount of antigen produced from one 120 cm2 bottle was sufficient to coat at least 50 microtitre plates. The indirect ELISA was governed by a monoclonal antibody (MAb) to bovine IgG and the competitive ELISA by a MAb to BVDV. Preliminary results indicate that this latter MAb binds to a 20 K protein, present in both cytopathogenic and non-cytopathogenic strains of BVDV. Cattle sera were examined from the field as well as sera collected from a heifer following natural infection. There was a good correlation between the results obtained by the two ELISA techniques and in serum neutralization test. For routine examination of bovine serum samples the indirect ELISA applying a MAb to bovine IgG is recommended.

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