The use of streptomycin resistance and chlorophyll deficiency for selection of somatic hybrids between nicotiana tabacum and nicotiana rustica

Toki, S.; Kameya, T.

Heredity 60(1): 1-5


ISSN/ISBN: 0018-067X
Accession: 006788996

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To prove the usefulness for somatic hybridisation of a Nicotiana tabacum L. double mutant (SA), which is both streptomycin resistant and chlorophyll deficient, protoplasts obtained from SA suspension cultures were fused via the dextran method with protoplasts isolated from N. rustica L. mesophyll cells. Prospective somatic hybrids were selected for streptomycin resistance and the ability to produce chlorophyll in regenerated plants. By using this selection system, green plants were recovered from 5 colonies. The hybrid nature of these plants was confirmed by morphological studies and isoelectric focusing of the Fraction 1 protein (RuBP carboxylase). The present results show evidence for the potential use of the double mutant in studies of somatic hybridisation.